Genetic diversity, antimicrobial resistance and extended-spectrum beta-lactamase type of Escherichia coli isolates from chicken, dog, pig and yak in Gansu and Qinghai Provinces, China

文献类型： 外文期刊

第一作者： Wang, Yining

作者： Wang, Yining;Zhou, Jianhua;Li, Xuerui;Ma, Lina;Cao, Xiaoan;Zhao, Lu;Jing, Wenxian;Lan, Xi;Li, Youquan;Gong, Xiaowei;Chen, Qiwei;Liu, Yongsheng;Hu, Wen;Stipkvits, Laszlo;Szathmary, Susan;Tarasiuk, Kazimierz;Pejsak, Zygmunt

作者机构：

关键词： Escherichia coli; Multilocus sequence typing; PCR phylogenetic typing; Drug resistance; Extended-spectrum beta-lactamase; ESBL

期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 影响因子：4.035； 五年影响因子：3.611 ）

ISSN： 2213-7165

年卷期： 2020 年 22 卷

页码：

收录情况： SCI

摘要： Objectives: In this study, the genetic diversity, phylogenetic grouping, antimicrobial resistance and extended-spectrum beta-lactamase (ESBL) types of Escherichia coli isolates from chickens, dogs, pigs and yaks in six prefectures of Gansu and Qinghai Provinces, China, were investigated. Methods: E. coli was isolated from diarrhoeic and healthy faecal samples. Multilocus sequence typing (MLST), phylogenetic grouping, antimicrobial resistance and ESBL profiles were investigated. Results: A total of 142 MLST sequence types (STs) were identified from 400 E. coli isolates. eBURST clustering analysis resolved the 142 STs into 19 clonal complexes (CCs) and 67 singletons. PCR phylogenetic typing determined the isolation rate of potentially pathogenic 62/D group isolates among all E. coli to be 12.5% from healthy animal samples and 17.5% from diarrhoeic samples. Antimicrobial susceptibility testing revealed 78 antimicrobial resistance patterns. E. coli resistance rates were highest to doxycycline, ampicillin and tetracycline, whereas polymyxin B and meropenem had the lowest resistance rates. All polymyxin B-resistant E. coli isolates were positive for the mcr-1 gene. A total of 62 ESBL-producing isolates were identified. The ESBL prevalence was 55.0% in diarrhoeic samplings and 5.6% in healthy animals. TEM (82.3%) was the predominant ESBL type, followed by CTM (43.5%) and SHV (19.4%). Conclusion: E. coli isolates in the study area have a high diversity of genetic and antimicrobial resistance patterns but a relatively low isolation rate of potentially pathogenic phylogroups. However, the somewhat high isolation rate of multidrug-resistant E. coli, particularly ESBL-producing isolates, requires continual surveillance of E. coli from animals in these areas. (C) 2020 Lanzhou Veterinary research institute ,CAAS. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.

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