Identification ofACTBGene as a Potential Safe Harbor Locus in Pig Genome

文献类型： 外文期刊

第一作者： Xiong, Youcai

作者： Xiong, Youcai;Han, Xiaosong;Zhang, Jinfu;Zhao, Guangxing;Wang, Zichang;Zhuang, Rongzhi;Nie, Xiongwei;Xie, Shengsong;Li, Changchun;Li, Xinyun;Liu, Xiangdong;Zhao, Shuhong;Ruan, Jinxue;Xie, Shengsong;Li, Changchun;Li, Xinyun;Liu, Xiangdong;Zhao, Shuhong;Li, Kui;Ruan, Jinxue

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关键词： Safe harbor; ACTB; CRISPR; Cas9; Homologous recombination; Pig

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN： 1073-6085

年卷期： 2020 年 62 卷 11-12 期

页码：

收录情况： SCI

摘要： Transgenic pigs play an important role in biomedicine and agriculture. The "safe harbor" locus maintains consistent foreign gene expression in cells and is important for transgenic pig generation. However, as only several safe harbor loci(Rosa26, pH11andPifs501) have been identified in pigs, meeting the needs of the insertion of various foreign genes is difficult. In this study, we develop a novel strategy for the efficient knock-in of gene-of-interest fragments into endogenous beta-actin(ACTB) gene via CRISPR/Cas9 mediated homologous recombination with normal expression ofACTB. Thus, we provide an alternative strategy to integrate exogenous genes into the pig genome that can be applied to agricultural breeding and biomedical models.

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