Visible to Near-Infrared Emission Ratiometric Fluorescent Probe for the Detection of Vanin-1 In Vivo

文献类型： 外文期刊

第一作者： Qian, Jing

作者： Qian, Jing;Wang, Jiemin;Cao, Ting;Zheng, Lei;Cao, Yuping;Qin, Wenwu;Qian, Jing;Wang, Jiemin;Cao, Ting;Zheng, Lei;Cao, Yuping;Qin, Wenwu;Teng, Zhidong;Guo, Huichen;Teng, Zhidong;Guo, Huichen;Zhang, Liang;Liu, Yun

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关键词： pantetheinase detection; NIR emission ratiometric; mice imaging; fast and efficient response; associated with diabetes

期刊名称：ACS SENSORS （ 影响因子：7.711； 五年影响因子：8.162 ）

ISSN： 2379-3694

年卷期： 2020 年 5 卷 9 期

页码：

收录情况： SCI

摘要： Pantetheinase (Vann -1) is an ectoenzyme, which involves the metabolic pathway of coenzyme A (CoA), and can decompose pantetheine into pantothenic acid (CoA precursor) and aminothiol cysteamine. Previous studies have revealed that Vanin-1 with essential biological functions is closely related to many diseases. However, the lack of simple and effective detection methods has severely hindered the further study of Vanin-l's physiological ftinctions. In this work, we have developed a nearinfrared (NIR) emission ratio fluorescent probe TMN-PA (I-645 nm/I-568 (nm)) that enables us to detect Vanin-1 rapidly (in 15 min) with a minimum detection limit of 0.37 ng/mL. What is more, this probe shows excellent potential in in situ real-time monitoring of the endogenous Vanin-1, contributing to further research on Vanm-1 and understanding its mechanisms in physiological pathology. To our knowledge, this probe is the first NIR emission ratio (I-645 nm/I-568 (nm)) fluorescent probe ever reported to monitor the activity of Vanin-1 in vivo.

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