Predicating the Effector Proteins Secreted byPuccinia triticinaThrough Transcriptomic Analysis and Multiple Prediction Approaches

文献类型： 外文期刊

第一作者： Zhang, Yue

作者： Zhang, Yue;Wei, Jie;Qi, Yue;Li, Jianyuan;Amin, Raheela;Yang, Wenxiang;Li, Jianyuan;Liu, Daqun

作者机构：

关键词： Puccinia triticina; effector proteins; BAX; qRT-PCR; RNA sequencing

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Wheat leaf rust caused byPuccinia triticinais one of the most common and serious diseases in wheat production. The constantly changing pathogens overcome the plant resistance toP. triticina. Plant pathogens secrete effector proteins that alter the structure of the host cell, interfere plant defenses, or modify the physiology of plant cells. Therefore, the identification of effector proteins is critical to reveal the pathogenic mechanism. We used SignalP v4.1, TargetP v1.1, TMHMM v2.0, and EffectorP v2.0 to screen the candidate effector proteins inP. triticinaisolates - KHTT, JHKT, and THSN. As a result, a total of 635 candidate effector proteins were obtained. Structural analysis showed that effector proteins were small in size (50AA to 422AA) and of diverse sequences, and the conserved sequential elements or clear common elements were not involved, regardless of their secretion from the pathogen to the host. There were 427 candidate effector proteins that contain more than or equal to 4 cysteine residues, and 339 candidate effector proteins contained the known motifs. Sixteen families, 9 domains, and 53 other known functional types were found in 186 candidate effector proteins using the Pfam search. Three novel motifs were found by MEME. Heterogeneous expression system was performed to verify the functions of 30 candidate effectors by inhibiting the programmed cell death (PCD) induced by BAX (the mouse-apoptotic gene elicitor) onNicotiana benthamiana. Hypersensitive response (HR) can be induced by the six effectors in the wheat leaf rust resistance near isogenic lines, and this would be shown by the method of transient expression throughAgrobacterium tumefaciensinfiltration. The quantitative reverse transcription PCR (qRT-PCR) analysis of 14 candidate effector proteins secreted afterP. triticinainoculation showed that the tested effectors displayed different expression patterns in different stages, suggesting that they may be involved in the wheat-P. triticinainteraction. The results showed that the prediction ofP. triticinaeffector proteins based on transcriptomic analysis and multiple bioinformatics software is effective and more accurate, laying the foundation of revealing the pathogenic mechanism ofPtand controlling disease.

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