Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJ Delta 1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4

文献类型： 外文期刊

第一作者： Dong, Weiwei

作者： Dong, Weiwei;Cai, Yuyuan;Xu, Zhilong;Fu, Biao;Chen, Qitong;Cui, Yuxin;Liang, Yunxiang;Peng, Nan;Zhao, Shumiao;Dong, Weiwei;Cai, Yuyuan;Xu, Zhilong;Fu, Biao;Chen, Qitong;Cui, Yuxin;Liang, Yunxiang;Peng, Nan;Zhao, Shumiao;Ruan, Zhiyong

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关键词： Quorum sensing; Quorum quenching; AHL lactonase AiiK; Lactobacillus casei MCJ Delta 1; Aeromonas hydrophila

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：5.328； 五年影响因子：5.588 ）

ISSN：

年卷期： 2020 年 19 卷 1 期

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收录情况： SCI

摘要： Background: Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an effective and promising way to prevent and control pathogens, driving it be the potential bio-control agents. In our previous studies, AHL lactonase AiiK was identified with many characteristics, and constitutive expression vector pELX1 was constructed to express heterologous proteins in Lactobacillus casei MCJ Delta 1 (L. casei MCJ Delta 1). In this study, recombinant strain pELCW-aiiK/L. casei MCJ Delta 1 (LcAiiK) and wild-typeAeromonas hydrophila (A. hydrophila) were co-cultured to test the QQ ability of LcAiiK againstA. hydrophila. Results: A cell wall-associated expression vector pELCW forL. casei MCJ Delta 1 was constructed. Localization assays revealed that the expressed AiiK was anchored at the surface layer of LcAiiK via vector pELCW-aiiK. LcAiiK (OD600 = 0.5) degraded 24.13 mu M of C-6-HSL at 2 h, 40.99 mu M of C-6-HSL at 12 h, and 46.63 mu M of C-6-HSL at 24 h. Over 50% LcAiiK cells maintained the pELCW-aiiKplasmid after 15 generations of cultivation without erythromycin. Furthermore, LcAiiK inhibited the swimming motility, extracellular proteolytic activity, haemolytic activity and biofilm formation ofA. hydrophila AH-1 and AH-4. Conclusion: The AHL lactonase AiiK is firstly and constitutively expressed at the surface layer ofL. casei MCJ Delta 1. LcAiiK displayed considerable AHL lactonase activity and great QQ abilities againstA. hydrophila AH-1 and AH-4 by attenuating their QS processes instead of killing them. Therefore, the LcAiiK can be exploited as an anti-pathogenic drug or a bio-control agent to control the AHL-mediated QS of pathogenic bacteria.

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