Genome- and Transcriptome-Wide Identification of C3Hs in Common Bean (Phaseolus vulgarisL.) and Structural and Expression-Based Analyses of Their Functions During the Sprout Stage Under Salt-Stress Conditions

文献类型： 外文期刊

第一作者： Zhang, Qi

作者： Zhang, Qi;Zhang, Wen-jing;Li, Wei-jia;Zhao, Hao-hao;Zhang, Shuo;Zhuang, Lin;Wang, Yu-xin;Zhang, Wen-Hui;Du, Ji-Dao;Yin, Zhen-gong;Du, Ji-Dao

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关键词： C3Hgene family; common bean; sprout stage; salt stress; gene expression analysis; transcriptome

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

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年卷期： 2020 年 11 卷

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收录情况： SCI

摘要： CCCH (C3H) zinc-finger proteins are involved in plant biotic and abiotic stress responses, growth and development, and disease resistance. However, studies onC3Hgenes inPhaseolus vulgarisL. (common bean) are limited. Here, 29 protein-encodingC3Hgenes, located on 11 different chromosomes, were identified inP. vulgaris. A phylogenetic analysis categorized thePvC3Hs into seven subfamilies on the basis of distinct features, such as exon-intron structure,cis-regulatory elements, and MEME motifs. A collinearity analysis revealed connections among thePvC3Hs in the same and different species. ThePvC3Hgenes showed tissue-specific expression patterns during the sprout stage, as assessed by real-time quantitative PCR (RT-qPCR). Using RNA-sequencing and RT-qPCR data,PvC3Hs were identified as being enriched through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses in binding, channel activity, and the spliceosome pathway. These results provide useful information and a rich resource that can be exploited to functionally characterize and understandPvC3Hs. ThesePvC3Hs, especially those enriched in binding, channel activity, and the spliceosome pathway will further facilitate the molecular breeding of common bean and provide insights into the correlations betweenPvC3Hsand salt-stress responses during the sprout stage.

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