Molecular characterization of 60S ribosomal protein L12 of E. tenella

文献类型： 外文期刊

第一作者： Liang, Shanshan

作者： Liang, Shanshan;Zhu, Shunhai;Zhao, Qiping;Yu, Yu;Dong, Hui;Wang, Qingjie;Wang, Haixia;Yu, Shuilan;Huang, Bing;Han, Hongyu;Liang, Shanshan;Yu, Yu

作者机构：

关键词： Avian coccidiosis; Eimeria tenella; 60S ribosomal protein

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN： 0014-4894

年卷期： 2020 年 217 卷

页码：

收录情况： SCI

摘要： This study analyzed the large-subunit (60S) ribosomal protein L12 of Eimeria tenella (Et60s-RPL12). A full-length cDNA was cloned, and the recombinant protein was expressed in E. coli BL21 and inoculated in rabbits to produce the polyclonal antibody. Quantitative real-time polymerase chain reaction and western blotting were used to analyze the transcription levels of Et60s-RPL12 and translation levels in different developmental stages of E. tenella. The results showed that the mRNA transcription level of Et60s-RPL12 was highest in second-generation merozoites, whereas the translation level was highest in unsporulated oocysts. Indirect immunofluorescence showed that Et60s-RPL12 was localized to the anterior region and surface of sporozoites, except for the two refractile bodies. As the invasion of DF-1 cells progressed, fluorescence intensity was increased, and Et60s-RPL12 was localized to the parasitophorous vacuole membrane (PVM). The secretion assay results using staurosporine indicated that this protein was secreted, but not from micronemes. The role of Et60s-RPL12 in invasion was evaluated in vitro. The results of the invasion assay showed that polyclonal antibody inhibited host cell invasion by the parasite, which reached about 12%. However, the rate of invasion was not correlated with the concentration of IgG.

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