Map-based cloning of a novel QTL qBN-1 influencing branch number in soybean [Glycine max (L.) Merr.]

文献类型： 外文期刊

第一作者： Lamlom, Sobhi F.

作者： Lamlom, Sobhi F.;Zhang, Xia;Fu, Jindong;Qiu, Li-Juan;Lamlom, Sobhi F.;Zhang, Yong;Su, Bohong;Wu, Haitao;Zhang, Bo

作者机构：

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2020 年 8 卷 5 期

页码：

收录情况： SCI

摘要： Branch number (BN) is an important agronomic attribute related to the plant architecture, adaptability, and yield of soybean. To date, few studies ofBNhave been conducted to elucidate its genetic background. We aimed to localize genetic factors affecting BN using segregating populations derived fromthe high-branching cultivar `Kennong24' (KN24) and the low-branching cultivar `Kenfeng19' (KF19). Composite interval mapping analysis detected a QTL (qBN-1) on chromosome 6 between the SSR markers BARCSOYSSR_06_0993 and BARCSOYSSR_06_1070 using an F2 population. To fine-map qBN-1, a RIL population was developed and genotyped with 14 SSRmarkers located in the QTL region. qBN-1 was localized to a 115.67-kb interval flanked by markers BARCSOYSSR_06_1048 and BARCSOYSSR_06_1053. The QTL was further confirmed using backcross populations of size 1305 (BC2F2 with KN24 as a recurrent parent) and 1712 (BC3F2 with KF19 as a recurrent parent). The fine-mapping region of qBN-1 contained only two candidate genes, Glyma.06G208800 and Glyma.06G208900, whose expression patterns were investigated by qRT-PCR. Compared to Glyma.06G208800 gene expression, Glyma.06G208900 showed the highest expression of the two genes and showed a significant difference in expression between high-and low-branching genotypes in either axillary meristem or shoot apical meristem, and showed opposite expression patterns in the two tissues at V4 and R1 stages. These results identify Glyma.06G208900 as a novel candidate gene controlling BN. Taken together, the results of this study provide a foundation for cloning and functional analysis of the qBN-1 gene and for the improvement of BN bymarker-assisted selection in soybean breeding. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

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