Characterization of L-Theanine Hydrolase in Vitro and Subcellular Distribution of Its Specific Product Ethylamine in Tea (Camellia sinensis)

文献类型： 外文期刊

第一作者： Fu, Xiumin

作者： Fu, Xiumin;Cheng, Sihua;Liao, Yinyin;Xu, Xinlan;Yang, Ziyin;Fu, Xiumin;Cheng, Sihua;Liao, Yinyin;Xu, Xinlan;Yang, Ziyin;Cheng, Sihua;Liao, Yinyin;Yang, Ziyin;Wang, Xinchao;Hao, Xinyuan;Xu, Ping;Dong, Fang;Yang, Ziyin

作者机构：

关键词： albino; Camellia sinensis; ethylamine; subcellular localization; tea; L-theanine hydrolase

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2020 年 68 卷 39 期

页码：

收录情况： SCI

摘要： L-Theanine has a significant role in the taste of tea (Camellia sinensis) infusions. Our previous research indicated that the lower L-theanine metabolism in ethylamine and L-glutamate is a key factor that explains the higher content of L-theanine in albino tea with yellow or white leaves, compared with that of normal tea with green leaves. However, the specific genes encoding L-theanine hydrolase in tea remains unknown. In this study, CsPDX2.1 was cloned together with the homologous Arabidopsis PDX2 gene and the recombinant protein was shown to catalyze L-theanine hydrolysis into ethylamine and L-glutamate in vitro. There were higher CsPDX2.1 transcript levels in leaf tissue and lower transcripts in the types of albino (yellow leaf) teas compared with green controls. The subcellular location of ethylamine in tea leaves was shown to be in the mitochondria and peroxisome using a nonaqueous fractionation method. This study identified the L-theanine hydrolase gene and subcellular distribution of ethylamine in tea leaves, which improves our understanding of the L-theanine metabolism and the mechanism of differential accumulation of L-theanine among tea varieties.

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