Fine mapping of a candidate gene for cool-temperature-induced albinism in ornamental kale

文献类型： 外文期刊

第一作者： Yan, Chenghuan

作者： Yan, Chenghuan;Peng, Liying;Zhang, Lei;Yan, Chenghuan;Qiu, Zhengming

作者机构：

关键词： Albino trait; Semi-dominant inheritance; BSR-seq; Cytochrome P450 gene; Ornamental kale

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background The symptoms of cool-temperature-induced chlorosis (CTIC) are widely existed in higher plants. Although many studies have shown that the genetic mechanism of CTIC is generally controlled by recessive genes in model plants, the dominant inheritance of albinism has not been reported thus far. Here, two CTIC mutants, Red Kamome and White Kamome, were utilized to analyse the inheritance of the albino trait in ornamental kale. The objective of this investigation is to fine-map the target locus and identify the most likely candidate genes for albinism. Results Genetic analysis revealed that the albinism in the inner leaves of ornamental kale followed semi-dominant inheritance and was controlled by a single locus in two segregating populations. BSR-seq in combination with linkage analysis was employed to fine-map the causal gene, namedAK(Albino Kale), to an approximate 60 kb interval on chromosome C03. Transcriptome data from two extreme pools indicated that the differentially expressed gene ofBol015404, which encodes a cytochrome P450 protein, was the candidate gene. TheBol015404gene was demonstrated to be upregulated in the albino leaves of ornamental kale by qPCR. Additionally, the critical temperature for the albinism was determined between 10 degrees C and 16 degrees C by gradient test. Conclusions Using two independent segregating populations, the albino mutants were shown to be controlled by one semi-dominant gene,AK, in ornamental kale. TheBol015404gene was co-segregated with albinism phenotypes, suggesting this unknown function P450 gene as the most likely candidate gene. The albino trait appeared caused by the low temperatures rather than photoperiod. Our results lay a solid foundation on the genetic control of albinism in ornamental kale.

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