CpG-ODN 2007 protects zebrafish (Danio rerio) againstVibrio vulnificusinfection

文献类型： 外文期刊

第一作者： Chen, Hua

作者： Chen, Hua;Zhang, Lijuan;Ke, Ling;Chen, Xu;Lin, Chentao;Lin, Chentao;Lin, Chentao

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关键词： CpG-ODN 2007; gene expression; Vibrio vulnificus; zebrafish (Danio rerio); ZF4 cells

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

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收录情况： SCI

摘要： Vibrio vulnificusis an aquatic pathogen that can cause primary sepsis and soft tissue infection. CpG oligodeoxynucleotides (CpG-ODN) are a type of essential immunomodulators, which can trigger or enhance immune responses in mammals, fish and humans. In this study, we evaluated the effect of CpG-ODN 2007 as a potential immunostimulant for zebrafish (Danio rerio) infected withV. vulnificusstrain FJ03-X2. Fish injected with the CpG-ODN 2007 showed a lower mortality rate compared with the fish that did not receive treatment; the survival rates of the CpG-ODN 2007-treated group and PBS-treated group were 85% and 57.9% respectively. Also, our in vitro results demonstrated that CpG-ODN 2007 could effectively reduce the toxicity ofV. vulnificusstrain FJ03-X2 to zebrafish embryonic fibroblast (ZF4) cells. Furthermore, we assessed innate immune-related genes (TNF, IL-1 beta, IFNg1-2, MyD88, lysozyme, IL-10, IL-22, IL-26 and TLRs) in expression patterns ofV. vulnificusstrain FJ03-X2 infected zebrafish or ZF4 cells with and without CpG-ODN 2007 treatment. We found that CpG-ODN 2007 could reduce the toxicity ofV. vulnificusto ZF4 cells and regulate the immune system to control the overexpression of specific genes. To sum up, our data indicated that CpG-ODN 2007 protects zebrafish againstVibrio vulnificusinfection.

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