The Twin-Arginine Translocation System Is Important for Stress Resistance and Virulence of Brucella melitensis

文献类型： 外文期刊

第一作者： Yan, Xin

作者： Yan, Xin;Hu, Sen;Yang, Yan;Xu, Da;Li, Huoming;Liu, Wenxing;He, Xijun;Li, Ganwu;Cai, Wentong;Bu, Zhigao;Hu, Sen;Cai, Wentong;Bu, Zhigao;Li, Ganwu

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关键词： Brucella melitensis; stress; translocated substrates; twin-arginine protein translocation; virulence

期刊名称：INFECTION AND IMMUNITY （ 影响因子：3.441； 五年影响因子：3.702 ）

ISSN： 0019-9567

年卷期： 2020 年 88 卷 11 期

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收录情况： SCI

摘要： Brucella, the causative agent of brucellosis, is a stealthy intracellular pathogen that is highly pathogenic to a range of mammals, including humans. The twin-arginine translocation (Tat) pathway transports folded proteins across the cytoplasmic membrane and has been implicated in virulence in many bacterial pathogens. However, the roles of the Tat system and related substrates in Brucella remain unclear. We report here that disruption of Tat increases the sensitivity of Brucella melitensis M28 to the membrane stressor sodium dodecyl sulfate (SDS), indicating cell envelope defects, as well as to EDTA. In addition, mutating Tat renders M28 bacteria more sensitive to oxidative stress caused by H2O2. Further, loss of Tat significantly attenuates B. melitensis infection in murine macrophages ex vivo. Using a mouse model for persistent infection, we demonstrate that Tat is required for full virulence of B. melitensis M28. Genome-wide in silk degrees prediction combined with an in vivo amidase reporter assay indicates that at least 23 proteins are authentic Tat substrates, and they are functionally categorized into solute-binding proteins, oxidoreductases, cell envelope biosynthesis enzymes, and others. A comprehensive deletion study revealed that 6 substrates contribute significantly to Brucella virulence, including an Lo-transpeptidase, an ABC transporter solute-binding protein, and a methionine sulfoxide reductase. Collectively, our work establishes that the Tat pathway plays a critical role in Brucella virulence.

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