Genome-Wide Identification, Characterization, and Regulation of RWP-RK Gene Family in the Nitrogen-Fixing Clade

文献类型： 外文期刊

第一作者： Wu, Zhihua

作者： Wu, Zhihua;Liu, Hong;Huang, Wen;Yi, Lisha;Qin, Erdai;Yang, Tiange;Qin, Rui;Wu, Zhihua;Liu, Hong;Huang, Wen;Yi, Lisha;Qin, Erdai;Yang, Tiange;Qin, Rui;Wang, Jing

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关键词： RWP-RK; nitrogen-fixing clade; co-expression network; evolution; nodulation

期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）

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年卷期： 2020 年 9 卷 9 期

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收录情况： SCI

摘要： RWP-RK is a plant-specific family of transcription factors, involved in nitrate response, gametogenesis, and nodulation. However, genome-wide characterization, phylogeny, and the regulation of RWP-RK genes in the nodulating and non-nodulating plant species of nitrogen-fixing clade (NFC) are widely unknown. Therefore, we identified a total of 292 RWP-RKs, including 278 RWP-RKs from 25 NFC species and 14 RWP-RKs from the outgroup,Arabidopsis thaliana. We classified the 292 RWP-RKs in two subfamilies: the NIN-like proteins (NLPs) and the RWP-RK domain proteins (RKDs). The transcriptome and phylogenetic analysis of RWP-RKs suggested that, compared toRKDgenes, theNLPgenes were just upregulated in nitrate response and nodulation. Moreover, nodule-specificNLPgenes of some nodulating NFC species may have a common ancestor (OG0002084) withAtNLPgenes inA. thaliana. Further, co-expression networks ofA.thalianaunder N-starvation and N-supplementation conditions revealed that there is a higher correlation between expression ofAtNLPgenes and symbiotic genes during N-starvation. InP. vulgaris, we confirmed that N-starvation stimulated nodulation by regulating expression ofPvNLP2, closely related to AtNLP6 and AtNLP7 with another common origin (OG0004041). Taken together, we concluded that different origins of theNLPgenes involved in both N-starvation response and specific expression of nodulation would contribute to the evolution of nodulation in NFC plant species. Our results shed light on the phylogenetic relationships ofNLPgenes and their differential regulation in nitrate response ofA. thalianaand nodulation of NFC.

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