Protective effects of astaxanthin on lipopolysaccharide-induced inflammation in bovine endometrial epithelial cells

文献类型： 外文期刊

第一作者： Wan, Fa-Chun

作者： Wan, Fa-Chun;Jin, Qing;Wei, Chen;Zhao, Hong-Bo;Zhang, Xiang-Lun;You, Wei;Liu, Xiao-Mu;Liu, Gui-Fen;Liu, Yi-Fan;Tan, Xiu-Wen;Wan, Fa-Chun;Jin, Qing;Wei, Chen;Zhao, Hong-Bo;Zhang, Xiang-Lun;You, Wei;Liu, Xiao-Mu;Liu, Gui-Fen;Liu, Yi-Fan;Tan, Xiu-Wen;Wan, Fa-Chun;Jin, Qing;Wei, Chen;Zhao, Hong-Bo;Zhang, Xiang-Lun;You, Wei;Liu, Xiao-Mu;Liu, Gui-Fen;Liu, Yi-Fan;Tan, Xiu-Wen;Wan, Fa-Chun;Zhang, Chen

作者机构：

关键词： astaxanthin; endometrial epithelial cells; oxidative stress; apoptosis; tight junction proteins

期刊名称：BIOLOGY OF REPRODUCTION （ 影响因子：4.285； 五年影响因子：4.307 ）

ISSN： 0006-3363

年卷期： 2020 年 102 卷 2 期

页码：

收录情况： SCI

摘要： Astaxanthin (AST), a natural antioxidant carotenoid, has been shown to exert anti-inflammatory effects. However, to our knowledge, no study has specifically addressed the potential protective effects of AST against bovine endometritis. The purpose of this study was to examine whether treatment with AST could protect endometrial epithelial cells against lipopolysaccharide (LPS)-induced inflammatory injury. Treatment of bovine endometrial (BEND) epithelial cell line with AST reduced LPS-induced production of interleukin-6 and tumor necrosis factor-alpha, increased the cellular activity of superoxide dismutase and catalase, decreased the proportion of apoptotic cells, and promoted the production of insulin-like growth factor and epithelial growth factor. The effects of AST were mediated through the downregulation of B-cell lymphoma 2 (Bcl-2) associated X, apoptosis regulator (Bax), and cleaved caspase-3 and through the upregulation of Bcl-2. Moreover, AST significantly increased the expression of the tight junction proteins (TJP) claudin, cadherin-1, and TJP1, which play an essential role in the maintenance of host endometrial defense barrier against pathogen infection. Collectively, these results demonstrated that treatment with AST protected against oxidative stress, prevented cell apoptosis, promoted BEND cells viability, and increased the production of growth factors, in addition to activating the endometrial defense barrier. Therefore, AST is a promising therapeutic agent for the prevention and treatment of endometritis. This finding is of utmost importance in the present times when the excessive use of antibiotics has resulted in the development of antibiotic-resistant bacteria. Summary sentence Astaxanthin counteracts LPS-induced inflammation, oxidative stress, apoptosis, and tight junction disassembly in bovine endometrial epithelial cells, and is a potential therapeutic agent against bovine endometritis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序