RPS5 interacts with the rabbit hemorrhagic disease virus 3 ' extremities region and plays a role in virus replication

文献类型： 外文期刊

第一作者： Guo, Hongyuan

作者： Guo, Hongyuan;Zhu, Jie;Miao, Qiuhong;Qi, Ruibin;Tang, Aoxing;Liu, Chuncao;Yang, Hongzao;Liu, Guangqing;Miao, Qiuhong;Yang, Hongzao;Yuan, Ligang

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关键词： Rabbit hemorrhagic disease virus; RPS5; RNA-protein interaction; Viral replication

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2020 年 249 卷

页码：

收录情况： SCI

摘要： Rabbit hemorrhagic disease virus (RHDV), a member of Caliciviridae family, causes a highly contagious disease in rabbits. The RHDV replication mechanism is poorly understood due to the lack of a suitable culture system in vitro. This study identified RHDV 5' and 3' extremities (Ex) RNA binding proteins from the rabbit kidney cell line RK-13 based on a pull-down assay by applying a tRNA scaffold streptavidin aptamer. Using mass spectrometry (MS), several host proteins were discovered which interact with RHDV 5' and 3' Ex RNA. The ribosomal protein S5 (RPS5) was shown to interact with RHDV 3' Ex RNA directly by RNA-pulldown and confocal microscopy. To further investigate the role of RPS5 in RHDV replication, small interfering RNAs for RPS5 and RPS5 eukaryotic expression plasmids were used to change the expression level of RPS5 in RK-13 cells and the results showed that the RHDV replication and translation levels were positively correlated with the expression level of RPS5. It was also verified that RPS5 promoted RHDV replication by constructing RPS5 stable overexpression cell lines and RPS5 knockdown cell lines. In summary, it has been identified that RPS5 interacted with the RHDV 3' Ex RNA region and played a role in virus replication. These results will help to understand the mechanism of RHDV replication.

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