Construction of Gossypium barbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

文献类型： 外文期刊

第一作者： Abid, Muhammad Ali

作者： Abid, Muhammad Ali;Wang, Peilin;Zhu, Tao;Liang, Chengzhen;Meng, Zhigang;Guo, Sandui;Zhang, Rui;Malik, Waqas

作者机构：

关键词： ethylmethanesulfonate; mutation breeding; mutant bank; germplasm resources; plant architecture; virescent phenotype

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 18 期

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收录情况： SCI

摘要： Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton. Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library. At M-6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

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