Identification and expression analysis of the WRKY gene family during different developmental stages in Lycium ruthenicum Murr. fruit

文献类型： 外文期刊

第一作者： Tiika, Richard John

作者： Tiika, Richard John;Wei, Jia;Ma, Rui;Ma, Yanjun;Tiika, Richard John;Wei, Jia;Yang, Hongshan;Cui, Guangxin;Duan, Huirong

作者机构：

关键词： Lycium ruthenicum; WRKY gene; Fruit developmental stages; Gene expression; Conserved motif; Transcriptome data

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2020 年 8 卷

页码：

收录情况： SCI

摘要： Background. The WRKY gene family, one of the major transcription factor families in plants, plays crucial regulatory roles in physiological and biological developmental processes, and the adaptation of plants to the environment. However, the systematic study of WRKY structure, expression profiling, and regulatory functions has not been extensively reported in Lycium ruthenicum, although these aspects have been comprehensively studied in most plant species. Methods. In this study, theWRKY genes were identified from a L. ruthenicum transcriptome database by using bioinformatics. The identification, phylogenetic analysis, zincfinger structures, and conserved motif prediction were extensively explored. Moreover, the expression levels of 23 selected genes with fragments per kilobase of exons per million mapped reads (FPKM) >5 were assayed during different fruit developmental stages with real-time quantitative polymerase chain reaction (RT-qPCR). Results. A total of 73 putative WRKY proteins in the L. ruthenicum transcriptome database were identified and examined. Forty-four proteins with the WRKY domain were identified and divided into three major groups with several subgroups, in accordance with those in other plant species. All 44 LrWRKY proteins contained one or two conserved WRKY domains and a zinc-finger structure. Conserved motif prediction revealed conservation of the WRKY DNA-binding domain in L. ruthenicum proteins. The selected LrWRKY genes exhibited discrete expression patterns during different fruit developmental stages. Interestingly, five LrWRKYs (-20, -21, -28, -30, and -31) were expressed remarkably throughout the fruit developmental stages. Discussion. Our results reveal the characteristics of the LrWRKY gene family, thus laying a foundation for further functional analysis of theWRKY family in L. ruthenicum.

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