The genome analysis of halotolerant Sphingobium yanoikuyae YC-XJ2 with aryl organophosphorus flame retardants degrading capacity and characteristics of related phosphotriesterase

文献类型： 外文期刊

第一作者： Wu, Wei

作者： Wu, Wei;Wang, Junhuan;Jia, Yang;Yan, Yanchun;Li, Xianjun;Reheman, Aikebaier;Wang, Dezhou

作者机构：

关键词： Sphingobium yanoikuyae; Genome sequencing; Phosphotriesterase; Aryl organophosphorus flame retardants

期刊名称：INTERNATIONAL BIODETERIORATION & BIODEGRADATION （ 影响因子：4.32； 五年影响因子：4.719 ）

ISSN： 0964-8305

年卷期： 2020 年 155 卷

页码：

收录情况： SCI

摘要： The genome of Sphingobium yanoikuyae YC-XJ2 with aryl organophosphorus flame retardants (aryl-OPFRs) degrading activity was sequenced and analyzed systematically. The gene sy-pte was cloned and expressed, and the molecular mechanism of aryl-OPFRs degradation was elucidated. Sy-PTE exhibited enzymatic properties of alkali, high temperature and salt resistance. The hydmlyzation of EHDPP by phosphotriesterase was first reported. The specific activity of Sy-PTE was 67.0 +/- 1.4 (U mg(-1)) for TPhP with k(cat)/K-m and V-max values of 4.8 +/- 0.6 x 10(3) (mM(-1).s(-1)) and 0.29 +/- 0.02 (mu M.s(-1)), K-m of 15.6 +/- 0.34 (mu M), k(cat) of 75.02 +/- 1.5 s(-1), and need no metal ion activator to assist. The structural comparison of the metal binding sites with Sb-PTE (PDB ID 5HRM) showed that the transition from uncharged Met-259 to positively charged Asn-259 in Sy-PET might affect the binding of Mn2+ to His-258 because of the same electrical properties, and the transition from Gly-473 without side chains in Sb-PTE to Ala-473 might cause steric hindrance in binding of Mn2+ to His-475. Based on enzymatic advantages, Sy-PTE should be a promising engineered enzyme in the future for bioremediation of aryl-OPFRs polluted sites.

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