文献类型： 外文期刊

第一作者： Qu, Cheng

作者： Qu, Cheng;Zhang, Biyun;Wang, Ran;Li, Fengqi;Luo, Chen;Kang, Zhiwei;Fang, Yong;Zhao, Haipeng

作者机构：

关键词： Spodoptera frugiperda; genome; sex pheromone gland; biosynthesis pathway

期刊名称：INSECTS （ 影响因子：3.139； 五年影响因子：3.285 ）

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年卷期： 2022 年 13 卷 12 期

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收录情况： SCI

摘要： Simple Summary The fall armyworm (FAW), Spodoptera frugiperda, is a serious worldwide agricultural pest, threatening food security and crop production. Sex pheromone lures are commonly used in population monitoring and biological control of FAWs. Although the sex pheromone components of the FAW have been successfully identified, there are no reports on the molecular mechanism of FAW sex pheromone biosynthesis. In this study, we identified a total of 99 genes related to the biosynthesis of sex pheromones from the S. frugiperda genome, which belonged to 11 families of genes. Based on gene expression patterns and phylogenetic analysis, several genes had PG-biased expression, indicating that they may play an important role in sex pheromone biosynthesis. These results could lay a solid foundation for understanding the molecular mechanisms of S. frugiperda sex pheromone biosynthesis and provide new targets for developing novel pest control methods based on disrupting sexual communication. Spodoptera frugiperda is an agricultural pest causing substantial damage and losses to commercial crops. Sex pheromones are critical for successful mating in Lepidoptera and have been used for monitoring and control of many pest species. The sex pheromone of S. frugiperda is known, but the genes involved in its biosynthesis have not been identified. We systematically studied 99 candidate sex pheromone genes in the genome of S. frugiperda including 1 acetyl-CoA carboxylase (ACC), 11 fatty acid synthases (FASs), 17 desaturases (DESs), 4 fatty acid transport proteins (FATPs), 29 fatty acyl-CoA reductases (FARs), 17 acetyl-CoA acetyltransferases (ACTs), 5 acyl-CoA dehydrogenase (ACDs), 3 enoyl-CoA hydratases (ECHs), 3 hydroxyacyl-CoA dehydrogenases (HCDs), 6 ethyl-CoA thiolases (KCTs), and 3 acyl-CoA-binding proteins (ACBPs). Based on the comparative transcriptome results, we found 22 candidate sex pheromone biosynthesis genes predominately expressed in pheromone glands (PGs) than abdomens without PGs including SfruFAS4, SfruFATP3, SfruACD5, SfruKCT3, SfruDES2, SfruDES5, SfruDES11, SfruDES13, SfruFAR1, SfruFAR2, SfruFAR3, SfruFAR6, SfruFAR7, SfruFAR8, SfruFAR9, SfruFAR10, SfruFAR11, SfruFAR14, SfruFAR16, SfruFAR29, SfruACT6, and SfruACT10. A combination of phylogenetic and tissue-specific transcriptomic analyses indicated that SfruDES5, SfruDES11, SfruFAR2, SfruFAR3, and SfruFAR9 may be key genes involved in the sex pheromone synthesis of S. frugiperda. Our results could provide a theoretical basis for understanding the molecular mechanisms of sex pheromone biosynthesis in S. frugiperda, and also provide new targets for developing novel pest control methods based on disrupting sexual communication.

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