FK506 Targets MoFpr1 to Modulate Autophagy and Ubiquitination, Inhibiting the Pathogenicity of Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Zhao, Wen-Hui

作者： Zhao, Wen-Hui;Yan, Jiong-Yi;Xie, Ke-Xin;Luo, Xi-Chun;Liao, Jian;An, Chun-Yue;Liu, Xiao-Hong;Yan, Jiong-Yi;Liang, Shuang;Zhu, Xue-Ming;Lin, Fu-Cheng;Wu, Xi-Yu;Qian, Hui;Lu, Jian-Ping;Khan, Irshad Ali

作者机构：

关键词： FK506; X-ray crystallography; riceblast; broad-spectrum antifungal activity

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 24 期

页码：

收录情况： SCI

摘要： Rice blast, caused by Magnaporthe oryzae, is a major threat to global rice production. This study explores the antifungal potential of the immunosuppressant FK506 and identifies its target protein, MoFpr1 (FK506-binding protein 1B). FK506 inhibited mycelial growth, appressorium formation, and pathogenicity of M. oryzae in an MoFpr1-dependent manner. Mechanistic analyses revealed that FK506 impairs autophagy and ubiquitination, supported by transcriptomic and metabolomic data. Structural studies using X-ray crystallography and site-directed mutagenesis confirmed the direct interaction between FK506 and MoFpr1, highlighting the importance of residues Gly95 and Ile97. Furthermore, FK506 demonstrated broad-spectrum antifungal activity against various plant pathogens and effectively controlled rice blast in laboratory, net-chamber, and field trials with minimal phytotoxicity. These findings position FK506 as a promising antifungal agent and offer insights into its molecular mechanism, suggesting its potential for sustainable plant disease management.

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