Alkaline hydrogen peroxide pretreatment combined with bio-additives to boost high-solids enzymatic hydrolysis of sugarcane bagasse for succinic acid processing

文献类型： 外文期刊

第一作者： Zhang, Jun

作者： Zhang, Jun;Li, Kuntai;Liu, Shucheng;Xu, Chao;Xu, Chao;Huang, Shushi;Zhang, Jun;Li, Kuntai;Liu, Shucheng

作者机构：

关键词： Lignocellulosic biomass; Succinic acid; Pretreatment; Enzymatic hydrolysis; Fermentation

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.889； 五年影响因子：11.139 ）

ISSN： 0960-8524

年卷期： 2022 年 345 卷

页码：

收录情况： SCI

摘要： Alkaline hydrogen peroxide (AHP) pretreatment of sugarcane bagasse (SCB) at mild conditions was optimized with response surface methodology (RSM), then enzymatic hydrolysis was performed at high-solids substrate loading (30 %, w/v), followed by fed-batch fermentation to convert the fermentable sugars into succinic acid (SA). Results showed the AHP pretreatment conditions of H2O2 concentration 5.5 % (v/v), solid-to-liquid ratio 0.08, pretreatment temperature 65 degrees C and time 5 h could achieve the highest sugar yield (74.3 %); both additives and fed-batch strategy were favored to boost enzymatic hydrolysis, the concentration and yield of total sugars reached to 195 g/L and 70 % with cellulase dosage of only 6 FPU/g dry biomass (DM); all glucose and xylose could be utilized after fed-batch fermentation, and the obtained concentration and yield of SA reached 41.4 g/L and 63.8 %. In summary, a SA conversion rate high to 0.29 g/g SCB raw material could be achieved via the developed process.

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