Knockdown of Sly-miR160a using short tandem target mimic (STTM) enhanced expression of auxin signaling genes and delayed postharvest ripening of tomato fruit
文献类型： 外文期刊
第一作者： Mekontso, Francine Ngaffo
作者： Mekontso, Francine Ngaffo;Wu, Shuhui;Fu, Ruizuo;Meng, Lanhuan;Song, Hongmiao;Xu, Xiangbin;Li, Wen;Wang, Qing;Li, Jiangkuo;Song, Hongmiao;Xu, Xiangbin
作者机构：
关键词： MiR160a; ARFs; Tomato; Ripening; Auxin signaling
期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：7.0； 五年影响因子：6.9 ）
ISSN： 0925-5214
年卷期： 2023 年 198 卷
页码：
收录情况： SCI
摘要： The miR160 is a highly conserved microRNA (miRNA) that regulates the auxin signaling pathway through negative regulation of auxin response factors (ARFs) and plays pivotal roles in fruit development. In the present study, the knockdown of miR160a using short tandem target mimic (STTM 160a) and overexpression of miR160a (miR160a-OE) transgenic tomato lines were generated to investigate the possible roles of miR160 in postharvest ripening tomato fruit. The results showed that the STTM 160a increased total chlorophyll content, reduced lycopene and total carotenoid accumulation, weight loss, and respiration rate while increasing firmness in to-mato fruit compared with the wild-type (WT) and miR160a-OE. Additionally, miR160a-OE lines exhibited a typical auxin hypersensitivity and postharvest ripening acceleration, whereas STTM 160a lines displayed an auxin hyposensitivity and postharvest ripening reduction. Moreover, the relative expression levels of ARF10A (Solyc11g069500.2.1), ARF10B (Solyc06g075150.3.1), ARF17 (Solyc11g013480.2.1), and Aux/IAA genes were up-regulated in STTM 160a lines. The results suggest that the knockdown of Sly-miR160a delays postharvest ripening in tomato fruit, and provide valuable information for genetic engineering in controlling developmental processes.
分类号：
- 相关文献
作者其他论文 更多>>
-
Transcriptomic, metabolomic, and physiological analysis of two varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) that differ in their storabilit
作者：Zhao, Keyan;Yuan, Shuzhi;Xu, Xiangbin;Shi, Junyan;Zuo, Jinhua;Yue, Xiaozhen;Su, Tongbing;Wang, Qing;Zhao, Keyan;Zhu, Xiaoqian;Xu, Xiangbin;Zhao, Keyan;Zhu, Xiaoqian;Yuan, Shuzhi;Xu, Xiangbin;Shi, Junyan;Zuo, Jinhua;Yue, Xiaozhen;Su, Tongbing;Wang, Qing
关键词：Chinese Cabbage; Metabolomic; Transcripomic; Ethylene; Chlorophyll; Methyl jasmonate
-
Advances in dual-functional packaging: Visual monitoring of food freshness using plant essential oils and pH-sensitive natural pigments
作者：Peng, Qiong;Bao, Feng;Tang, Min;Zhong, Feifei;Li, Wen;Deng, Jing;Lin, Qinlu;Peng, Qiong;Li, Wen;Lin, Qinlu;Peng, Qiong;Li, Wen;Lin, Qinlu;Zhong, Feifei;Yan, Mingli;Yan, Mingli;Zuberi, Zavuga;Zuberi, Zavuga
关键词：Dual-functional packaging; Food freshness; Natural pigments; Plant essential oils; Visual monitoring
-
Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection
作者：Wu, Huiliang;Yin, Jiyuan;Jiang, Peng;Wang, Linchuan;Wang, Qing;Li, Siming;Yan, Han;Wang, Hao;Ding, Zhaoyang;Li, Pengfei;Chen, Bo;Wang, Qing;Wang, Qing
关键词：Grass carp reovirus; VP4; Oral immunization; L. lactis; Immunogenicity; Protective efficacy
-
Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus x female Channa maculata ) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)
作者：Deng, Huiling;Zheng, Shucheng;Li, Yingying;Mo, Xubing;Zhao, Jian;Yin, Jiyuan;Shi, Cunbin;Wang, Qing;Wang, Yingying;Deng, Huiling;Zheng, Shucheng;Wang, Qing;Wang, Yingying
关键词：CAMK cell line; Hybrid snakehead; Virology; Hybrid snakehead rhabdovirus
-
Revealing the ACE receptor binding properties and interaction mechanisms of salty oligopeptides from Stropharia rugosoannulata mushroom by molecular simulation and antihypertensive evaluation
作者：Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Liu, Peng;Li, Zhengpeng;Yang, Yan;Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Liu, Peng;Li, Zhengpeng;Yang, Yan;Li, Wen;Ma, Haile;Wang, Jinbin
关键词：
-
A Vis/NIR device for detecting moldy apple cores using spectral shape features
作者：Liu, Haoling;Wei, Ziyuan;Lu, Miao;Zhao, Juan;Gao, Pan;Hu, Jin;Liu, Haoling;Wei, Ziyuan;Lu, Miao;Gao, Pan;Zhao, Juan;Hu, Jin;Li, Jiangkuo;Gao, Pan;Hu, Jin
关键词：Apple; Moldy core; Portable device; Vis/NIR; Spectral shape features
-
Multi-omic analysis of the extension of broccoli quality during storage by folic acid
作者：Zhao, Yaqi;Shi, Junyan;Yuan, Shuzhi;Yue, Xiaozhen;Shi, Wenlin;Yan, Zhicheng;Xu, Dongying;Zuo, Jinhua;Wang, Qing;Zhao, Yaqi;Zhao, Yaqi;Feng, Bihong;Shi, Wenlin
关键词：Folic acid; Broccoli; DNA methylation; Transcriptomic; Metabolomic
