Novel epitopes identified from Tembusu virus NS3 protein induce cytotoxic T lymphocyte response

文献类型： 外文期刊

第一作者： Liu, Keshu

作者： Liu, Keshu;Chen, Liu;Yun, Tao;Ni, Zheng;Ye, Weicheng;Zhang, Cun;Sun, Yangyang

作者机构：

关键词： TMUV; CTL epitope; ELISpot; Cytotoxic activity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.246； 五年影响因子：3.565 ）

ISSN： 0378-1135

年卷期： 2022 年 271 卷

页码：

收录情况： SCI

摘要： Since 2010, Tembusu virus (TMUV) has spread widely in China, causing huge economic losses to the poultry industry. Due to the infectious and zoonotic nature of flaviviruses, their potential threat to public health is of great concern. Cellular immune responses usually play a critical role in combating viral infections. To study the molecular basis of cell immunity induced by TMUV, 14 cytotoxic T lymphocyte (CTL) epitope peptides of TMUV antigen E, NS1 and NS3 were predicted by bioinformatics tools. Their abilities to induce cellular immune responses were determined by IFN-gamma ELISpot assay, and 4 peptides were found to exhibit highly significant responses upon stimulation. In addition, the cytotoxic activity induced by the epitope peptides was assessed by lactate dehydrogenase (LDH) release assay. Finally, among these peptides, we identified two murine TMUV NS3derived H-2d-restricted CTL epitopes in BALB/c mice, which could be used to further study of epitope vaccines against TMUV infection.

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