Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus)

文献类型： 外文期刊

第一作者： Yang, Chang-Geng

作者： Yang, Chang-Geng;Sun, Bing;Liu, Shan-Shan;Chen, Song-Lin;Yang, Chang-Geng;Wang, Xian-Li;Zhang, Bo

作者机构：

关键词： Akirin;Japanese flounder;NF-kappa B;Yeast two-hybrid assay;Immunity;HEPN;C1q

期刊名称：BMC MOLECULAR BIOLOGY （ 影响因子：2.946； 五年影响因子：3.192 ）

ISSN： 1471-2199

年卷期： 2013 年 14 卷

页码：

收录情况： SCI

摘要： A member of the NF-kappa B signaling pathway, PoAkirin1, was cloned from a full-length cDNA library of Japanese flounder (Paralichthys olivaceus). The full-length cDNA comprises a 5'UTR of 202 bp, an open reading frame of 564 bp encoding a 187-amino-acid polypeptide and a 521-bp 3'UTR with a poly (A) tail. The putative protein has a predicted molecular mass of 21 kDa and an isoelectric point (pl) of 9.22. Amino acid sequence alignments showed that PoAkirin1 was 99% identical to the Scophthalmus maximus Akirin protein (ADK27484). Yeast two-hybrid assays identified two proteins that interact with PoAkirin1: PoHEPN and PoC1q. The cDNA sequences of PoHEPN and PoC1q are 672 bp and 528 bp, respectively. Real-time quantitative reverse-transcriptase polymerase chain reaction analysis showed that bacteria could induce the expressions of PoAkirin1, PoHEPN and PoC1q. However, the responses of PoHEPN and PoC1q to the bacterial challenge were slower than that of PoAkirin1. To further study the function of PoAkirin1, recombinant PoAkirin1 and PoHEPN were expressed in Escherichia coli and would be used to verify the PoAkirin1-PoHEPN binding activity. These results identified two proteins that potentially interact with PoAkirin1 and that bacteria could induce their expression.

分类号：

  • 相关文献

[1]Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in Turbot (Scophthalmus maximus). Yang, Chang-Geng,Liu, Shan-Shan,Sun, Bing,Wang, Na,Chen, Song-Lin,Yang, Chang-Geng,Sun, Bing,Wang, Xian-Li. 2013

[2]Arabidopsis heat stress transcription factors A2 (HSFA2) and A3 (HSFA3) function in the same heat regulation pathway. Li, Xiao-dong,Wang, Xiao-li,Cai, Yi-Ming,Wu, Jia-hai,Mo, Ben-tian,Yu, Er-ru.

[3]Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Liu, YG,Chen, SL,Li, J,Li, BF. 2006

[4]Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus). Chen, SL,Wang, ZJ,Xu, MY,Gui, JF. 2006

[5]Effects of dietary supplementation of A3 alpha-peptidoglycan on innate immune responses and defense activity of Japanese flounder (Paralichthys olivaceus). Zhou, J,Song, XL,Huang, J,Wang, XH. 2006

[6]Production and confirmation of clones using gynogenesis in Japanese flounder. Liu, Haijin,Liu, Yongxin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2011

[7]Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Liu, YG,Chen, SL,Li, BF. 2005

[8]Major histocompatibility complex class IIB allele polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Zhang, Y. X.,Chen, S. L.,Liu, Y. G.,Sha, Z. X.,Liu, Z. J.. 2006

[9]Production and verification of heterozygous clones in Japanese flounder, Paralichthys olivaceus by microsatellite marker. Liu, Haijin,Liu, Yongxin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2011

[10]Tissue-specific accumulation of cadmium and its effects on antioxidative responses in Japanese flounder juveniles. Cao, Liang,Huang, Wei,Shan, Xiujuan,Dou, Shuozeng,Huang, Wei,Shan, Xiujuan,Ye, Zhenjiang.

[11]Genome-wide SNP identification for the construction of a high-resolution genetic map of Japanese flounder (Paralichthys olivaceus): applications to QTL mapping of Vibrio anguillarum disease resistance and comparative genomic analysis. Shao, Changwei,Liu, Yang,Wang, Lei,Tian, Yongsheng,Chen, Songlin,Shao, Changwei,Liu, Yang,Wang, Lei,Tian, Yongsheng,Chen, Songlin,Shao, Changwei,Sakamoto, Takashi,Niu, Yongchao,Xie, Zhiyuan,Tai, Shuaishuai,Rastas, Pasi,Li, Hengde,Jiang, Yong.

[12]Genetic verification of doubled haploid Japanese flounder, Paralichthys olivaceus by genotyping telomeric microsatellite loci. Liu, Yongxin,Liu, Haijin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2012

[13]Efficacy of Vibrio anguillarum antigen administered by intraperitoneal injection route in Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel). Li, J,Gao, DM,Wang, Q,Wang, JY,Wang, QY. 2005

[14]Estimation of genetic parameters for growth traits of Japanese flounder Paralichthys olivaceus using an animal model. Liu, Yong-Xin,Liu, Hai-Jin,Wang, Gui-Xing,Wang, Yu-Fen,Si, Fei,Sun, Zhao-Hui,Zhang, Xiao-Yan,Wang, Jia-Di. 2011

[15]Influence of epistatic segregation distortion loci on genetic marker linkages in Japanese flounder. Zhao, Jingli,Gao, Jin,Yang, Runqing,Han, Dandan,Shi, Kuntao,Wang, Li. 2018

[16]Molecular cloning, structure and expressional profiles of two novel single-exon genes (PoCCR6A and PoCCR6B) in the Japanese flounder (Paralichthys olivaceus). Wang, Lei,Zhang, Yong-zhen,Xu, Wen-teng,Jia, Xiao-dong,Chen, Song-lin.

[17]Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder. Liu, Yi,Zhang, Xiaoyan,Si, Fei,Sun, Zhaohui,Wang, Guixing,Wang, Yufen,Liu, Yi,Liu, Yongxin,Liu, Yingjie,Yang, Runqing,Liu, Haijin.

[18]Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin,Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin.

[19]Design and synthesis of pregnenolone/2-cyanoacryloyl conjugates with dual NF-kappa B inhibitory and anti-proliferative activities. Song, Jia-Li,Zhu, Kong-Kai,Yang, Fei-Fei,Liu, Xi-Gong,Zhang, Yu-Ying,Jiang, Cheng-Shi,Zhang, Hua,Zhang, Juan,Longo, Joao Paulo Figueiro,Muehlmann, Luis Alexandre,Zhang, Juan,Longo, Joao Paulo Figueiro,Muehlmann, Luis Alexandre,Azevedo, Ricardo Bentes,Liu, Chang-Liang,Liu, Chao,Guo, Yue-Wei. 2017

[20]Molecular characterization and expression analysis of the I kappa B gene from pearl oyster Pinctada fucata. Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Qiu, Lihua,Su, Tianfeng,Wu, Kaichang,Li, Youning,Zhu, Caiyan,Zhang, Dianchang. 2009

作者其他论文 更多>>

微信小程序