RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata

文献类型： 外文期刊

第一作者： Liu, Junna

作者： Liu, Junna;Guo, Mujuan;Li, Zhaoyang;Pan, Huipeng;Nanda, Satyabrata;Zhou, Xuguo;Zhang, Youjun;Yang, Chunxiao

作者机构：

关键词： Henosepilachna vigintioctopunctata; Dietary RNAi; Feeding disorders; Pros alpha 2; Biopesticide

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 195 卷

页码：

收录情况： SCI

摘要： Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Pros alpha 2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvPros alpha 2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/mu L. Ingestion of the bacterially expressed dsHvPros alpha 2 caused high mortality in both larvae and adults. Moreover, silencing of HvPros alpha 2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvPros alpha 2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAibased control of H. vigintioctopunctata.

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