Efficient acquisition of high-purity cyanidin-3-O-glucoside from mulberry fruits: An integrated process of ATPS whole-cell transformation and semi-preparative HPLC purification

文献类型： 外文期刊

第一作者： Li, Yi-Tong

作者： Li, Yi-Tong;Huang, Ting;Wang, Jin-Zheng;Yan, Cheng-Hai;Gong, Lu-Chan;Wu, Fu-An;Wang, Jun;Gong, Lu-Chan;Wu, Fu-An;Wang, Jun

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关键词： Aqueous two-phase system; Cyanidin-3-O-glucoside; Cyanidin-3-O-rutinoside; Whole cell; Mulberry red pigment; Semi-preparative HPLC

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 404 卷

页码：

收录情况： SCI

摘要： As a nutritious fruit, mulberry is an ideal source of high-quality cyanidin-3-O-glucoside (C3G) with various biological activities. However, the difficult separation process of high-purity C3G leads to its high price. To rapidly prepare high-purity C3G, cyanidin-3-O-rutinoside is converted to C3G by direct hydrolysis of rhamnose bond using a whole-cell catalyst containing alpha-rhamnosidase. Combined with an aqueous two-phase system, a coupling reaction separation system was established. Two monomers were successfully separated by semi -preparative high performance liquid chromatography (semi-preparative HPLC). The conversion of C3G cata-lyzed by whole-cells in the PEG/Na2SO4 system increased from 47.11 % to 66.56 %, compared with the EtOH/ (NH4)2SO4 system, and the whole-cell activity remained above 50 % after five rounds of reuse. Meanwhile, the purity of C3G was increased to 99 % via the semi-preparative HPLC purification and identified by MS. Thus, an integrated process of whole-cell-catalyzed conversion and product peak cutting partition collection provides a novel strategy for efficient biomanufacturing of high-purity C3G.

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