An Investigation of the Relationship between Cyniclomyces guttulatus and Rabbit Diarrhoea

文献类型： 外文期刊

第一作者： Shi, Tuanyuan

作者： Shi, Tuanyuan;Sun, Hongchao;Fu, Yuan;Zhou, Yongxue;Liu, Yan;Bao, Guolian;Yan, Xinlei;Han, Wenying;Hao, Lili;Suo, Xun

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关键词： rabbit; diarrhoea; Cyniclomyces guttulatus; Eimeria intestinalis; opportunistic pathogen

期刊名称：PATHOGENS （ 影响因子：3.492； 五年影响因子：4.066 ）

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年卷期： 2021 年 10 卷 7 期

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收录情况： SCI

摘要： Cyniclomyces guttulatus is usually recognised as an inhabitant of the gastrointestinal (GI) tract in rabbits. However, large numbers of C. guttulatus are often detected in the faeces of diarrhoeic rabbits. The relationship of C. guttulatus with rabbit diarrhoea needs to be clearly identified. In this study, a C. guttulatus Zhejiang strain was isolated from a New Zealand White rabbit with severe diarrhoea and then inoculated into SPF New Zealand white rabbits alone or co-inoculated with Eimeria intestinalis, another kind of pathogen in rabbits. Our results showed that the optimal culture medium pH and temperature for this yeast were pH 4.5 and 40-42 degrees C, respectively. The sequence lengths of the 18S and 26S ribosomal DNA fragments were 1559 bp and 632 bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from dogs and 100% homology with the 26S ribosomal sequence of DPA-CGR1 and CGDPA-GP1 isolates from rabbits and guinea pigs, respectively. In animal experiments, the C. guttulatus Zhejiang strain was not pathogenic to healthy rabbits, even when 1 x 10(8) vegetative cells were used per rabbit. Surprisingly, rabbits inoculated with yeast showed a slightly better body weight gain and higher food intake. However, SPF rabbits co-inoculated with C. guttulatus and E. intestinalis developed more severe coccidiosis than rabbits inoculated with C. guttulatus or E. intestinalis alone. In addition, we surveyed the prevalence of C. guttulatus in rabbits and found that the positive rate was 83% in Zhejiang Province. In summary, the results indicated that C. guttulatus alone is not pathogenic to healthy rabbits, although might be an opportunistic pathogen when the digestive tract is damaged by other pathogens, such as coccidia.

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