Highly Sensitive and Rapid Screening Technique for the Detection of Organophosphate Pesticides and Copper Compounds Using Bifunctional Recombinant TrxA-PvCarE1

文献类型： 外文期刊

第一作者： Cao, Jing

作者： Cao, Jing;Wang, Miao;She, Yongxin;Zheng, Lufei;Jin, Fen;Shao, Yunling;Wang, Jing;Cao, Jing;Wang, Miao;She, Yongxin;Zheng, Lufei;Jin, Fen;Shao, Yunling;Wang, Jing;Abd El-Aty, A. M.;Abd El-Aty, A. M.

作者机构：

关键词： enzyme inhibition; pesticide; rapid detection; bifunctional recombinant; molecular docking

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 9 期

页码：

收录情况： SCI

摘要： Enabling the detection of organophosphate pesticide (OP) residues through enzyme inhibition-based technology is crucial for ensuring food safety and human health. However, the use of acetylcholinesterase, the primary target enzyme for OPs, isolated from animals in practical production poses challenges in terms of sensitivity and batch stability. To address this issue, we identified a highly sensitive and reproducible biorecognition element, TrxA-PvCarE1, derived from red kidney beans and successfully overexpressed it in Escherichia coli. The resulting recombinant TrxA-PvCarE1 exhibited remarkable sensitivity toward 10 OPs, surpassing that of commercial acetylcholinesterase. Additionally, this approach demonstrated the capability to simultaneously detect copper compounds with high sensitivity, expanding the range of pesticides detectable using the traditional enzyme inhibition method. Spiking recovery tests conducted on cowpea and carrot samples verified the suitability of the TrxA-PvCarE1-based technique for real-life sample analysis. In summary, this study highlights a promising comprehensive candidate for the rapid detection of pesticide residues.

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