Effects of combined addition of 3-nitrooxypropanol and vitamin B12 on methane and propionate production in dairy cows by in vitro-simulated fermentation

文献类型： 外文期刊

第一作者： Liu, Zihao

作者： Liu, Zihao;Wang, Kun;Nan, Xuemei;Yang, Liang;Wang, Yue;Zhang, Fan;Cai, Meng;Zhao, Yiguang;Xiong, Benhai

作者机构：

关键词： 3-nitrooxypropanol; rumen microbiota; vitamin B12; propionate formation; methanogenesis

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：3.5； 五年影响因子：4.2 ）

ISSN： 0022-0302

年卷期： 2023 年 106 卷 1 期

页码：

收录情况： SCI

摘要： The compound 3-nitrooxypropanol (3-NOP) is a promising methane inhibitor, which performs well in inhibiting methane emission and does not affect ani-mal feed intake and digestibility. However, it causes a significant increase in hydrogen production while sup-pressing methane emission, resulting in a waste of feed energy. Vitamin B12 is a key factor in the propionate production pathway and thus plays an important role in regulating the hydrogen utilization pathway. In this study, the effects of 3-NOP combined with vitamin B12 supplementation on rumen fermentation and microbial compositional structure in dairy cattle were investigat-ed by simulating rumen fermentation in vitro. Experi-ments were performed using a 2 x 2-factorial design: two 3-NOP levels (0 or 2 mg/g dry matter) and 2 vita-min B12 levels (0 or 2 mg/g dry matter). Three experi-ments were performed, each consisting of 4 treatments, 4 replicates, and 4 blanks containing only inoculum. The combined supplementation of 3-NOP and vitamin B12 reduced methane emission by 12% without affect-ing dry matter digestibility. The combined addition of 3-NOP and vitamin B12 significantly increased the concentration of propionate and reduced the concentra-tion of acetate and the acetate to propionate ratio. At the bacterial level, 3-NOP increased the relative abun-dances of Christensenellaceae_R-7_group and Lachno-spiraceae_NK3A20_group. Vitamin B12 increased the relative abundances of unclassified_f__Prevotellaceae and Prevotellaceae_UCG-003 and decreased the rela-tive abundance of Lachnospiraceae_NK3A20_group. At the archaeal level, the combination of 3-NOP and vi-tamin B12 increased the relative abundances of Metha-nobrevibacter_ sp._ Abm4, OTU1125, and OTU95 and decreased the relative abundances of uncultured_methanogenic_archaeon_g__Methanobrevibacter, OTU1147, OTU1056, and OTU55. The results indicated that 3-NOP combined with vitamin B12 could alleviate ru-men hydrogen emission and enhance the inhibition of methane emission compared with 3-NOP alone.

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