Acute chlorothalonil exposure had the potential to influence the intestinal barrier function and micro-environment in mice

文献类型： 外文期刊

第一作者： Tao, Huaping

作者： Tao, Huaping;Tao, Huaping;Wang, Juntao;Bao, Zhiwei;Jin, Yuanxiang;Xiao, Yingping;Xiao, Yingping

作者机构：

关键词： Chlorothalonil; Intestinal barrier function; Gut microbiota; Microenvironment; Mice

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）

ISSN： 0048-9697

年卷期： 2023 年 894 卷

页码：

收录情况： SCI

摘要： The intestinal barrier maintains intestinal homeostasis and metabolism and protects against harmful pollutants. Some en-vironmental pollutants seriously affect intestinal barrier function. However, it remains unclear whether or how chlorothalonil (CTL) impacts the intestinal barrier function in animals. Herein, 6-week-old male mice were acutely exposed to different CTL concentrations (100 and 300 mg/kg BW) via intragastric administration once a day for 7 days. Histopathol-ogical examination revealed obvious inflammation in the mice' colon and ileum. Most notably, CTL exposure increased the intestinal permeability, particularly in the CTL-300 group. CTL exposure reduced the secretion of colonic epithelial mucus and changed the transcription levels of genes bound up with ion transport and ileal antimicrobial peptide (AMP) secretion, indicating intestinal chemical barrier damage. The results of terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay and Ki67 staining revealed abnormal apoptosis and increased intestinal epithelial cell proliferation, suggest-ing that CTL exposure led to cytotoxicity and inflammation. The results of 16S rRNA sequencing revealed that CTL exposure altered the intestinal microbiota composition and reduced its diversity and richness in the colon contents. Thus, acute CTL exposure affected the different intestinal barrier-and gut microenvironment-related endpoints in mice.

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