Vitellogenin from planthopper oral secretion acts as a novel effector to impair plant defenses

文献类型： 外文期刊

第一作者： Ji, Rui

作者： Ji, Rui;Fu, Jianmei;Li, Jing;Wang, Lu;Yang, Shiying;Tian, Tian;Wang, Lihua;Ju, Jiafei;Guo, Huifang;Fang, Jichao;Shi, Yu;Jing, Maofeng;Wang, Lu;Yang, Shiying;Tian, Tian;Wang, Lihua;Dou, Daolong;Liu, Bin;Hoffmann, Ary A.;Zhu-Salzman, Keyan

作者机构：

关键词： effector; planthopper; plant-insect interaction; rice OsWRKY71; vitellogenin

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.152； 五年影响因子：10.476 ）

ISSN： 0028-646X

年卷期： 2021 年 232 卷 2 期

页码：

收录情况： SCI

摘要： Vitellogenin (Vg) is a well-known nutritious protein involved in reproduction in nearly all oviparous animals, including insects. Recently, Vg has been detected in saliva proteomes of several piercing-sucking herbivorous arthropods, including the small brown planthopper (Laodelphax striatellus, SBPH). Its function, however, remains unexplored. We investigated the molecular mechanism underlying SBPH orally secreted Vg-mediated manipulation of plant-insect interaction by RNA interference, phytohormone and H2O2 profiling, protein-protein interaction studies and herbivore bioassays. A C-terminal polypeptide of Vg (VgC) in SBPH, when secreted into rice plants, acted as a novel effector to attenuate host rice defenses, which in turn improved insect feeding performance. Silencing Vg reduced insect feeding and survival on rice. Vg-silenced SBPH nymphs consistently elicited higher H2O2 production, a well-established defense mechanism in rice, whereas expression of VgC in planta significantly hindered hydrogen peroxide (H2O2) accumulation and promoted insect performance. VgC interacted directly with the rice transcription factor OsWRKY71, a protein which is involved in induction of H2O2 accumulation and plant resistance to SBPH. These findings indicate a novel effector function of Vg: when secreted into host rice plants, this protein effectively weakened H2O2-mediated plant defense through its association with a plant immunity regulator.

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