A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene

文献类型： 外文期刊

第一作者： Zhang, Baohong

作者： Zhang, Baohong;Wang, Hongmei;Liu, Fang;Wang, Qinglian

作者机构：

关键词： Cotton; Transgene; Bioassay; Antibiotics; Herbicides; 2,4-D; In vivo; In vitro

期刊名称：TRANSGENIC COTTON, 2 EDITION: Methods and Protocols

ISSN： 1064-3745

年卷期： 2019 年 1902 卷

页码：

收录情况： SCI

摘要： Determining transgenic events is a critical step for obtaining transgenic plants as well as the later stage of application. Traditional methods, such as Northern blotting and qRT-PCR, for determining transgenic events require radioactively labeled substrates, expensive instruments, or long-time commitments, which results in lab and time-consuming as well as expensive costs. Those methods also require destroying the transgenic events. In this chapter, we present a simple and rapid method for determining transgenic cotton plants in both laboratory and field conditions. This method is based on the sensitivity of transgenic and non-transgenic plants to a specific chemical, such as antibiotics or herbicides. This method will facilitate the screening of transgenic events, save time, reduce cost, and speed up the application of transgenic technology on cotton breeding and production. More importantly, this is a nondestructive bioassay method; the transgenic plants can be transferred into greenhouse or field for the later study after the detection process.

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