LncRNA CA13/EFTUD2/CA13 caused crossed beak by regulating the mandibular calcification in chicken

文献类型： 外文期刊

第一作者： Wu, Min

作者： Wu, Min;Li, Xinxin;Xu, Junjie;Fan, Yihao;Wu, Xinglin;Liu, Xuelu;Su, Bofei;Wang, Dehe;Chen, Yifan;Hao, Erying;Chen, Hui;Shi, Lei;Chen, Jilan;Su, Dan;Bai, Hao

作者机构：

关键词： Chicken; Crossed beak; Mandible; Calcification; LncRNA CA13/EFTUD2/CA13

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 8 期

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收录情况： SCI

摘要： Crossed beak is a beak deformity that reduces feeding and drinking in affected chickens, thereby diminishing chicken production performance. Previous studies have shown that long non-coding RNA CA13 (lncRNA CA13), as an upstream regulator of carbonic anhydrase 13 (CA13), exhibited down-regulated expression in the lateral mandibular condyles of the short bone branch in cross-beaked chickens along with CA13. The study investigated the expression characteristics of lncRNA CA13 and its regulatory relationship with CA13 and elongation factor Tu GTP-binding domain-containing protein 2 (EFTUD2), laying the groundwork for the molecular mechanisms of lncRNA CA13 in chicken mandibular mineralization. The length of lncRNA CA13, amplified by 5 ' and 3 ' rapid amplification of cDNA ends (RACE) technique, was 2380 bp. Expression of lncRNA CA13 was significantly higher in mandibular condylar tissues compared to other tissues (P < 0.01). Using a successfully constructed osteoblastinduced differentiation model, orange calcified nodules were observed under the microscope after alizarin red staining, and the expressions of CA13, lncRNA CA13, runt-related transcription factor 2 (RUNX2), and alkaline phosphatase (ALP) were also detected to be significantly higher than those before induction (P < 0.01; P < 0.05). Integrated RNA affinity pull-down assay (RNA pull-down) combined with multiple bioinformatic analysis methods, as well as RNA binding protein immunoprecipitation assay (RIP), identified EFTUD2 as a transcription factor involved in the cellular mineralization process. The oe-lncRNA CA13 vector was constructed with an overexpression efficiency of 95.85 %, and the sh-lncRNA CA13 vector had an interference efficiency of 54.58 %. Overexpression of lncRNA CA13 significantly upregulated EFTUD2 and downregulated CA13; simultaneous interference with lncRNA CA13 significantly downregulated EFTUD2 and upregulated CA13. Thus, the lncRNA CA13/EFTUD2/CA13 axis plays a key role in the development of crossed beak by regulating the calcification process in the chicken mandible.

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