ORFV can carry TRAP gene expression via intracellular CRISPR/Cas9 gene editing technology

文献类型： 外文期刊

第一作者： Yu, YongZhong

作者： Yu, YongZhong;Zhang, Fan;Duan, Xuyang;Yang, ChaoQun;Cui, YuDong;Yu, Li

作者机构：

关键词： Orf virus; Staphylococcus aureus; TRAP; CRISPR; Cas9 technique; ORFV-TRAP recombinant virus

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：3.1； 五年影响因子：2.4 ）

ISSN： 0166-0934

年卷期： 2023 年 312 卷

页码：

收录情况： SCI

摘要： Orf is an acute and highly contracted human and animal infection caused by orf virus (ORFV), which mainly affects sheep, goats, and other species. Clinically, opportunistic or conditional pathogens such as Staphylococcus aureus (S. aureus) are often detected in cases of orf, which greatly increases the risk of disease progression and clinical death. It has been reported that TRAP gene products of S. aureus can broadly influence bacterial life and pathogenicity in vivo, and introduction of exogenous TRAP genes may help to inhibit the proliferation of bacteria. In order to achieve the combined control of ORFV and S. aureus, a novel approach to design a S. aureus TRAP gene vaccine using a live attenuated ORFV vector is proposed. In this study, CRISPR/Cas9 gene editing technology was used to disable vascular endothelial growth factor E of ORFV (VEGF-v) and introduced TRAP gene into this position. TRAP gene expression was detected in keratinocytes infected with recombinant virus. The construction and experimental verification of recombinant ORFV (ORFV-v/TRAP) will provide a reference for indepth studies on the prevention and control of mixed infectious disease.

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