A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice

文献类型： 外文期刊

第一作者： Tang, Xiaoqian

作者： Tang, Xiaoqian;Zhang, Qi;Zhang, Zhaowei;Tang, Xiaoqian;Zhang, Qi;Zhang, Zhaowei;Tang, Xiaoqian;Zhang, Qi;Zhang, Zhaowei;Tang, Xiaoqian;Zhang, Qi;Tang, Xiaoqian;Zhang, Qi;Tang, Xiaoqian;Catanante, Gaelle;Isabel Pividori, Maria;Marty, Jean-Louis

作者机构：

关键词： patulin; aptamer; lateral flow assay; on-site; apple juice

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.743； 五年影响因子：5.972 ）

ISSN：

年卷期： 2022 年 12 卷 2 期

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收录情况： SCI

摘要： Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B-1 (AFB(1)), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice.

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