Immulectin-2 from the ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae), modulates cellular and humoral responses against infection

文献类型： 外文期刊

第一作者： Wu, Pei-Pei

作者： Wu, Pei-Pei;Shu, Rui-Hao;Gao, Xin-Xin;Li, Miao-Miao;Zhang, Ji-Hong;Zhang, Huan;Qin, Qi-Lian;Zou, Zhen;Meng, Qian;Wu, Pei-Pei;Gao, Xin-Xin;Li, Miao-Miao;Zou, Zhen;Shu, Rui-Hao

作者机构：

关键词： Chinese cordyceps; C-type lectin; Entomopathogenic fungi; Melanization; Encapsulation; Phagocytosis

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.605； 五年影响因子：3.833 ）

ISSN： 0145-305X

年卷期： 2022 年 133 卷

页码：

收录情况： SCI

摘要： C type-lectins constitute a large family of pattern recognition receptors, playing important roles in insect immune defenses. Thitarodes xiaojinensis larvae showed distinct immune features after Ophiocordyceps sinensis, Cordyceps militaris, or Beauveria bassiana infection. Based on transcriptome and immunoblot analysis, we found that immulectin-2 (IML2) was induced after T. xiaojinensis larvae were infected by C. militaris or B. bassiana but maintained at a low level after larvae injected with O. sinensis or Ringer's buffer. Recombinant IML2 (rIML2) could promote melanization, encapsulation, phagocytosis, and hemocyte aggregation in vitro. RNA interference with IML2 induced a significant reduction in the transcript levels of various antimicrobial peptides. Importantly, we found that the abundance of O. sinensis blastospores coated with rIML2 dramatically decreased in the host hemolymph. Overall, this study demonstrated that T. xiaojinensis IML2 modulates cellular and humoral responses to entomopathogenic fungi, broadening our view of the immune interaction between O. sinensis and its host.

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