Expression of Proliferation Cell Nuclear Antigen (PCNA) in Peripheral Erythrocytes of Triploid Rainbow Trout Oncorhynchus mykiss (Walbaum)

文献类型： 外文期刊

第一作者： Liu, Yi

作者： Liu, Yi;Fan, Zhaoting;Liu, Haijin;Wang, Bingqian

作者机构：

关键词： Rainbow trout;triploid;PCNA;immature erythrocyte;erythroid cells;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 23 期

页码：

收录情况： SCI

摘要： The proliferation of erythroid cells in. peripheral blood was detected by anti-PCNA monoclonal antibody (PC10) with a means of immunocytochemistry in. diploid and triploid rainbow trout. The rate of PCNA positive erythroid cells in triploidy peripheral blood was detected by flow cytometry. The erythrocytes which were positive for PCNA were confined in the cells with high nuclear-cytoplasmic ratio. About 6.4% erythrocytes were found positive for PCNA in triploid rainbow trout while only 0.4% in. diploids. And the PCNA positive rate of the erythrocytes was 0.37% in. diploids and 8.39% in triploids in average. PCNA, a proliferating marker is found to be expressed only in immature cells in the peripheral erythrocytes of triploid rainbow trout This suggests that peripheral erythrocytes with the ability of proliferating are immature cells.

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