Induction of 2n pollen with colchicine during microsporogenesis in Phalaenopsis

文献类型： 外文期刊

第一作者： Wu, Ting

作者： Wu, Ting;Zhao, Xin;Yang, Shuhua;Yang, Jiahui;Zhu, Jun;Kou, Yaping;Ge, Hong;Jia, Ruidong;Wu, Ting;Yu, Xiaonan

作者机构：

关键词：   meiosis stage; flower bud growth; 2n pollen; colchicine

期刊名称：BREEDING SCIENCE （ 影响因子：2.014； 五年影响因子：2.716 ）

ISSN： 1344-7610

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收录情况： SCI

摘要： The induction of 2n pollen is an important technique for breeding polyploid plants. Here, we observed meio??? sis in the pollen mother cells (PMCs) of six Phalaenopsis cultivars and attempted to induce 2n pollen. The meiotic stage was related to flower bud length. During meiosis, Phalaenopsis cultivars with flower widths of approximately 20???40 mm and 50???60 mm had bud lengths of approximately 3???8 mm and 5???13 mm, respec??? tively. The duration of meiosis ranged from 4.2 to 14 d. This was the first study to characterize meiosis of the PMCs of Phalaenopsis. The natural generation frequency of 2n pollen varied from 0.68% to 1.78%. Meiotic stage and colchicine concentration significantly affected the induction of 2n pollen. The most effective treat??? ment for obtaining 2n pollen was 0.05% colchicine in the leptotene to zygotene stage for 3 d, which achieved a 2n pollen frequency of 10.04%.

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