Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)

文献类型： 外文期刊

第一作者： Yuan, Yanjiao

作者： Yuan, Yanjiao;Liu, Shufang;Bai, Cuicui;Liu, Hongbo;Zhuang, Zhimeng;Yuan, Yanjiao;Bai, Cuicui;Liu, Hongbo

作者机构：

关键词： golden cuttlefish;Sepia esculenta;microsatellite;population structure

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1661-6596

年卷期： 2012 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)(13)-enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni's correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta.

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