Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1(3

文献类型： 外文期刊

第一作者： Xu, Mengmeng

作者： Xu, Mengmeng;Huang, Mengmeng;Wang, Guodong;Han, Jingzhe;Niu, Xinxin;Han, Jinze;Yu, Hangbo;Zhang, Yulong;Liu, Runhang;Wu, Ziwen;Cui, Hongyu;Zhang, Yanping;Wang, Suyan;Liu, Yongzhen;Chen, Yuntong;Duan, Yulu;Gao, Yulong;Qi, Xiaole;Xu, Mengmeng;Han, Jingzhe;Han, Jinze;Li, Liuan;Cui, Hongyu;Zhang, Yanping;Wang, Suyan;Liu, Yongzhen;Chen, Yuntong;Duan, Yulu;Gao, Yulong;Qi, Xiaole

作者机构：

关键词： Pro-IL-1(3; Chicken; Monoclonal antibody; Detection; Antigenic epitope

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 2 期

页码：

收录情况： SCI

摘要： Pro-IL-1(3 is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1(3, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1(3 monoclonal antibody (mAb). The secreted mAb 1E12 can recognize exogenous or endogenous chicken pro-IL-1(3 by Western blot and IFA techniques, and for the first time, a novel antigen epitope 13SSLSEETFY21 of chicken pro-IL-1(3 recognized by this mAb was identified. This study not only provides an important tool for the detection and research of chicken pro-IL-1(3, but also has significant implications for understanding the antigenic structure and biochemical characteristics of chicken pro-IL-1(3.

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