Differential occurrence and strain composition of potato viruses between two major seed potato production zones in China suggest the need for different management strategies for the zones
文献类型: 外文期刊
第一作者: Gao, Yanling
作者: Gao, Yanling;Cheng, Shengqun;Lu, Wenhe;Gao, Yanling;Fan, Guoquan;Zhang, Wei;Shen, Yu;Zhang, Shu;Bai, Yanju;Qiu, Cailing;Nie, Xianzhou
作者机构:
关键词: China; occurrence; potato; strains; viruses; Chine; pomme de terre; souches; virus
期刊名称:CANADIAN JOURNAL OF PLANT PATHOLOGY ( 影响因子:2.0; 五年影响因子:2.0 )
ISSN: 0706-0661
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Viral disease is one of the major reasons leading to yield and quality reduction of potato production. To gain better knowledge about seed potato quality in China, the occurrences of six common viruses, including potato leafroll virus (PLRV) and potato viruses A (PVA), M (PVM), S (PVS), X (PVX) and Y (PVY), were investigated in two of China's four potato production zones (specifically, Zone I and Zone IV) from 2013 to 2016. A total of 1231 random tuber samples (Zone I, n = 571; Zone IV, n = 660) and 532 symptomatic leaf samples (Zone I, n = 375; Zone IV, n = 157) were collected in seed potato and commercial potato fields and tested for the viruses by reverse transcription (RT)-PCR (tuber samples) and/or enzyme-linked immunosorbent assay (ELISA; leaf samples). The results showed that virus-free tubers accounted for 79.7% and 52.0% in seed tuber samples from zones I and IV, respectively. All of the six viruses analysed were present in both zones I and IV, but occurred at different frequencies, with PVY and PVS being the predominant viruses in zones I and IV, respectively. Moreover, 44.0% and 39.8% of the virus-positive samples in zones I and IV, respectively, were found to be infected with more than one virus. Strain typing of PVS and PVY demonstrated the existence of similar strains of PVY (i.e. PVYO, PVYNTNb, PVYN:O, PVYN-Wi, PVY - (SYR-I and -II) and of PVS (i.e. PVSO and PVSA) in both zones. For PVY, recombinant strains were predominant, with SYR-II (27.7%) being the most abundant strain in Zone I and SYR-I (53.3%) being the predominant strain in Zone IV. PVSO was the predominant strain in Zone I whereas PVSA was the dominant strain in Zone IV. This study revealed not only the high incidence of viruses in both seed and commercial potatoes in China, but also the differential occurrence and strain composition of the viruses between two major production zones in China for the first time. In addition, the study suggests the need for different management strategies for different production zones to enhance the quality of seed potatoes in China. Les maladies virales sont une des principales causes de la reduction du rendement et de la qualite chez la pomme de terre. Afin d'acquerir de meilleures connaissances sur la qualite des pommes de terre de semence en Chine, les occurrences de six virus courants, y compris le virus de l'enroulement de la pomme de terre (VEP) ainsi que les virus A (PVA), M (PVM), S (PVS), X (PVX) et Y (PVY), ont ete etudies, de 2013 a 2016, dans deux des quatre zones productrices de pommes de terre de Chine (en particulier, Zone I et Zone IV). En tout, 1 231 echantillons aleatoires de tubercules (Zone I, n = 571; Zone IV, n = 660) et 532 echantillons de feuilles symptomatiques (Zone I, n =3 75; Zone IV, n = 157) ont ete collectes dans des champs commerciaux de pommes de terre de semence et de pommes de terre, et testes pour les virus par PCR par transcriptase inverse (RT-PCR) (echantillons de tubercules) et/ou par essai d'immunoabsorption enzymatique (ELISA) (echantillons de feuilles). Les resultats ont montre que les tubercules exempts de virus constituaient 79,7% et 52,0% des echantillons des pommes de terre de semence des Zones I et IV, respectivement. Les six virus analyses ont ete trouves dans les deux zones, mais a des frequences differentes, PVY et PVS predominant dans les zones I et IV, respectivement. De plus, 44,0% et 39,8% des echantillons porteurs de virus dans les zones I et IV, respectivement, etaient infectes par plus d'un virus. L'identification des souches de PVS et de PVY a demontre l'existence de souches semblables de PVY (c.-a-d. PVYO, PVYNTNb, PVYN:O, PVYN-Wi, PVY [SYR-I, et -II]) et de PVS (c.-a-d. PVSO et PVSA), dans les deux zones. Chez PVY, les souches recombinantes predominaient, SYR-II (27,7%) etant la souche la plus abondante dans la Zone I et SYR-I (53,3%) predominant dans la Zone IV. PVSO etait la souche predominante dans la Zone I, tandis que PVSA predominait dans la Zone IV. Cette etude a non seulement revele le taux eleve de virus dans les productions commerciales de pommes de terre de semence et de pommes de terre en Chine, mais egalement, pour la premiere fois, l'occurrence differentielle et la composition des souches dans les deux principales zones. En outre, l'etude met en lumiere le besoin de strategies differentes pour diverses zones de production afin d'ameliorer la qualite des pommes de terre de semence en Chine.
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