Kill two birds with one stone: colorimetric/fluorescence immunosensor based on Au@Pt nanozyme for sensitive detection of imidacloprid

文献类型： 外文期刊

第一作者： Zhai, Rongqi

作者： Zhai, Rongqi;Chen, Ge;Liu, Beibei;Liu, Guangyang;Zhang, Xing;Liu, Junjiang;Xu, Xiaomin;Zhang, Yanguo;Xu, Donghui;Liu, Beibei;Wang, Jing;Jin, Maojun

作者机构：

关键词： Colorimetric detection; Fluorescence; Immunoassay; Imidacloprid; Au@Pt

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 10 期

页码：

收录情况： SCI

摘要： Gold-platinum (Au@Pt) nanozymes with high catalytic activity and stability were designed to improve the stability of the enzyme-linked immunosorbent assay (ELISA), and a two-mode signal output was used to enhance the sensitivity and confidence of the assay. This study reports the two-mode signal output based on Au@Pt nanozyme to catalyzed 3,3 ',5,5 '-tetramethylbenzidine (TMB) reaction. Oxidized 3,3 ',5,5 '-tetramethylbenzidine (ox-TMB) has wide absorption spectrum, providing excellent optical density capabilities and fluorescence quenching. The detection limits of imidacloprid were 0.88 mu g/L and 1.14 mu g/L in colorimetric and fluorescence modes, respectively. Multiple-mode strategy improves detection accuracy, increases the confidence of experimental results, and broadens detection modes. Two modes can meet the requirements of accurate and flexible multi-mode sensing in different application situations.

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