In vivo assembly in tobacco cells to elucidate and engineer the biosynthesis of 4-hydroxydihydrocinnamaldehyde from Gloriosa superba

文献类型： 外文期刊

第一作者： Xiong, Zhiqiang

作者： Xiong, Zhiqiang;Ding, Zhuoying;Sun, Jingyi;Cong, Hanqing;Sun, Huapeng;Qiao, Fei;Xiong, Zhiqiang;Ding, Zhuoying;Sun, Jingyi;Cong, Hanqing;Sun, Huapeng;Qiao, Fei;Xiong, Zhiqiang;Ding, Zhuoying;Sun, Jingyi;Jiang, Xuefei

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关键词： 4-HDCA; Biosynthesis pathway; Heterologous reconstruction; Tobacco cells; Stable transformation

期刊名称：PLANT CELL REPORTS （ 影响因子：4.5； 五年影响因子：6.1 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 10 期

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收录情况： SCI

摘要： The precursor, 4-hydroxydihydrocinnamaldehyde (4-HDCA), participates in the biosynthesis of the carbon skeleton of colchicine, which is derived from L-phenylalanine. However, one hypothesis proposed that 4-HDCA is synthesized by sharing the early part of the monolignol pathway in G. superba. In this study, we validated this prediction and identified the enzymatic functions involved in this pathway. GsDBR1 is a crucial enzyme to illustrate 4-HDCA diverging from monolignol pathway, we first confirmed its reductase activity on 4-coumaraldehyde, an important intermediate compound in monolignol biosynthesis. Then, the biochemical function of recombinant enzymes belonging to the other four families were verified to elucidate the entire process of 4-HDCA biosynthesis from L-phenylalanine. After reconstruction, the 4-HDCA was 78.4 ng/g with fresh weight (FW) of transgenic tobacco cells, and the yield increased to 168.22 ng/gFW after improved treatment with methyl jasmonate (MeJA). The elucidation of 4-HDCA biosynthesis sharing the monolignol pathway supplemented the biosynthesis of colchicine in G. superba, and the production of 4-HDCA in tobacco cells provides an important step in the development of plant cell cultures as heterologous bio-factories for secondary metabolite production.

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