RNA N-6-methyladenosine modification suppresses replication of rice black streaked dwarf virus and is associated with virus persistence in its insect vector

文献类型： 外文期刊

第一作者： Tian, Shuping

作者： Tian, Shuping;Wu, Nan;Zhang, Lu;Wang, Xifeng

作者机构：

关键词： N-6 methylation of adenosine (m(6)A); persistent transmission; replication; rice black streaked dwarf virus; small brown planthopper (Laodelphax striatellus); virus titre

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2021 年 22 卷 9 期

页码：

收录情况： SCI

摘要： N-6 methylation of adenosine (m(6)A) was recently discovered to play a role in regulating the life cycle of various viruses by modifying viral and host RNAs. However, different studies on m(6)A effects on the same or different viruses have revealed contradictory roles for m(6)A in the viral life cycle. In this study, we sought to define the role of m(6)A on infection by rice black streaked dwarf virus (RBSDV), a double-stranded RNA virus, of its vector small brown planthopper (SBPH). Infection by RBSDV decreased the level of m(6)A in midgut cells of SBPHs. We then cloned two genes (LsMETTL3 and LsMETTL14) that encode m(6)A RNA methyltransferase in SBPHs. After interference with expression of the two genes, the titre of RBSDV in the midgut cells of SBPHs increased significantly, suggesting that m(6)A levels were negatively correlated with virus replication. More importantly, our results revealed that m(6)A modification might be the epigenetic mechanism that regulates RBSDV replication in its insect vector and maintains a certain virus threshold required for persistent transmission.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序