A naphthimide fluorescent probe for the detection of selenols in selenium-enriched Tan sheep

文献类型： 外文期刊

第一作者： Liu, Yuning

作者： Liu, Yuning;Feng, Xiaohui;Meng, Qingshi;Zhu, Jiawei;Jia, Xueting;Zhao, Qingyu;Tang, Chaohua;Yu, Yanan;Zhang, Junmin;Liu, Yuning;Feng, Xiaohui;Meng, Qingshi;Zhu, Jiawei;Jia, Xueting;Zhao, Qingyu;Tang, Chaohua;Yu, Yanan;Zhang, Junmin

作者机构：

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 373 卷

页码：

收录情况： SCI

摘要： An "off-on" fluorescent probe, Nap-DNB, which is based on naphthimide, was designed and developed for the detection of biological selenols in vitro. We have adopted a combination of a low-pH detection environment and reaction sites that are more difficult to destroy to avoid the interference of a large number of biological thiols in biological samples. Nap-DNB can completely respond to selenocysteine within 15 mins, with a detection limit of 92 nM. Nap-DNB was successfully used for the detection of selenols in the serum, liver, and longissimus dorsi of selenium-enriched Tan sheep. Through comparison, we found that the detection of selenols by the Nap-DNB is similar to that by thioredoxin reductase and glutathione peroxidase in a commercial kit method. Nap-DNB can be used for the detection of selenols in selenium-enriched Tan sheep.

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