Effects of Insulin on Proliferation, Apoptosis, and Ferroptosis in Primordial Germ Cells via PI3K-AKT-mTOR Signaling Pathway

文献类型： 外文期刊

第一作者： Ye, Liu

作者： Ye, Liu;Liu, Xin;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Ye, Liu;Liu, Xin;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Ye, Liu;Liu, Xin;Jin, Kai;Niu, Yingjie;Zuo, Qisheng;Chen, Guohong;Li, Bichun;Song, Jiuzhou;Han, Wei;Li, Bichun

作者机构：

关键词： insulin; PGCs; proliferation; apoptosis; ferroptosis; PI3K-AKT-mTOR

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 10 期

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收录情况： SCI

摘要： Primordial germ cells (PGCs) are essential for the genetic modification, resource conservation, and recovery of endangered breeds in chickens and need to remain viable and proliferative in vitro. Therefore, there is an urgent need to elucidate the functions of the influencing factors and their regulatory mechanisms. In this study, PGCs collected from Rugao yellow chicken embryonic eggs at Day 5.5 were cultured in media containing 0, 5, 10, 20, 50, and 100 mu g/mL insulin. The results showed that insulin regulates cell proliferation in PGCs in a dose-dependent way, with an optimal dose of 10 mu g/mL. Insulin mediates the mRNA expression of cell cycle-, apoptosis-, and ferroptosis-related genes. Insulin at 50 mu g/mL and 100 mu g/mL slowed down the proliferation with elevated ion content and GSH/oxidized glutathione (GSSG) in PGCs compared to 10 mu g/mL. In addition, insulin activates the PI3K/AKT/mTOR pathway dose dependently. Collectively, this study demonstrates that insulin reduces apoptosis and ferroptosis and enhances cell proliferation in a dose-dependent manner via the PI3K-AKT-mTOR signaling pathway in PGCs, providing a new addition to the theory of the regulatory role of the growth and proliferation of PGC in vitro cultures.

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