Rhizobium sophorae is the dominant rhizobial symbiont of Vicia faba L. In North China

文献类型： 外文期刊

第一作者： Zhang, Junjie

作者： Zhang, Junjie;Li, Shuo;Wang, Nan;Zhang, Junjie;Yang, Tao;Zong, Xuxiao;Brunel, Brigitte;Andrews, Mitchell;Wang, Entao

作者机构：

关键词： Faba bean; Symbiosis; Genetic diversity; Rhizobia; Rhizobium; Symbiovar viciae

期刊名称：SYSTEMATIC AND APPLIED MICROBIOLOGY （ 影响因子：4.064； 五年影响因子：3.835 ）

ISSN： 0723-2020

年卷期： 2022 年 45 卷 1 期

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收录情况： SCI

摘要： Faba bean (Vicia faba L.) is a major introduced grain-legume crop cultivated in China. In this study, rhizobia that nodulated faba bean grown in soils from three sites in North China (Hebei Province) were isolated and characterized. Firstly, isolates were categorized into genotypes by ribosomal IGS PCR-RFLP analysis, then representatives of the different IGS genotypes were further identified by phylogenetic analyses of 16S rRNA, housekeeping (atpD, recA) and nodulation (nodC) gene sequences. Rhizobial distribution based on the IGS genotype was related to the different soil physicochemical features by redundancy analysis. IGS typing and phylogenetic analyses of 16S rRNA and concatenated housekeeping gene sequences affiliated the 103 rhizobial strains isolated into four Rhizobium species/genospecies. A total of 69 strains of 3 IGS types were assigned to R. sophorae, 20 isolates of 5 IGS types to R. changzhiense and 9 isolates of 3 IGS types to R. indicum. The representative strain of the five remaining isolates (1 IGS type) was clearly separated from all Rhizobium type strains and was most closely related to defined genospecies according to the recently described R. leguminosarum species complex. Rhizobium sophorae strains (67% of total isolates) were common in all sites and shared an identical nodC sequence typical of faba bean symbionts belonging to symbiovar viciae. In this first study of rhizobia nodulating faba bean in Hebei Province, China, R. sophorae was found to be the dominant symbiont in contrast to other countries. (c) 2021 Elsevier GmbH. All rights reserved.

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