Exploration of transcriptional regulation network between buffalo oocytes and granulosa cells and its impact on different diameter follicles

文献类型： 外文期刊

第一作者： Xu, Xiaoxian

作者： Xu, Xiaoxian;Cui, Kuiqing;Luo, Xier;Liu, Qingyou;Xu, Xiaoxian;Jiang, Hancai;Wang, Dong;Rehman, Saif ur;Li, Zhipeng;Song, Xinhui;Cui, Kuiqing;Liu, Qingyou;Yang, Chunyan

作者机构：

关键词： Buffalo; Different diameter follicles; Granulosa cells; Oocytes; Transcriptome sequencing

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundBuffalo is a globally important livestock species, but its reproductive performance is relatively low than cattles. At present, dominant follicle development specific process and mechanistic role of follicular growth related genes in water buffaloes are not well understood. Therefore, we comprehensively performed transcriptomics of granulosa cells and oocytes from different-sized follicles in water buffalo to identify key candidate genes that influence follicle development and diameter, and further explored the potential regulatory mechanisms of granulosa cells and oocytes in the process of water buffalo follicle development.ResultsIn this study, we found918 granulosa cell transcripts and 1401 oocyte transcripts were correlated in follicles of different diameters, and the expression differences were significant. Subsequent enrichment analysis of the co-expressed differentially expressed transcripts identified several genes targeted by long non-coding RNAs (lncRNAs) and associated with follicular development. Notably, the upregulation of BUB1 regulated by MSTRG.41325.4 and interactive action of SMAD2 and SMAD7 might have key regulatory role in follicular development. Additionally, we also detected key differentially expressed genes that potentially influence follicular hormone metabolism and growth, like ID2, CHRD, TGIF2 and MAD2L1, and constructed an interaction network between lncRNA transcripts and mRNAs.ConclusionsIn summary, this study preliminarily revealed the differences in gene expression patterns among buffalo follicles of different sizes and their potential molecular regulatory mechanisms. It provides a new perspective for exploring the mechanism of buffalo follicular dominance and improving buffalo reproductive performance.

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