Recognition elements based on the molecular biological techniques for detecting pesticides in food: A review

文献类型: 外文期刊

第一作者: Zhao, Mingqi

作者: Zhao, Mingqi;Wang, Miao;Zhu, Yongan;Cao, Jing;She, Yongxin;Cao, Zhen;Wang, Jing;Zhao, Mingqi;Wang, Miao;Zhu, Yongan;Cao, Jing;She, Yongxin;Cao, Zhen;Wang, Jing;Zhang, Xiaoguang;Zhang, Xiaoguang;Li, Guangyue;Abd El-Aty, A. M.;Abd El-Aty, A. M.

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关键词: Molecular biology techniques; recognition elements; biosensor; immunoassay technology

期刊名称:CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION ( 影响因子:11.208; 五年影响因子:12.104 )

ISSN: 1040-8398

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收录情况: SCI

摘要: Excessive use of pesticides can cause contamination of the environment and agricultural products that are directly threatening human life and health. Therefore, in the process of food safety supervision, it is crucial to conduct sensitive and rapid detection of pesticide residues. The recognition element is the vital component of sensors and methods for fast testing pesticide residues in food. Improper recognition elements may lead to defects of testing methods, such as poor stability, low sensitivity, high economic costs, and waste of time. We can use the molecular biological technique to address these challenges as a good strategy for recognition element production and modification. Herein, we review the molecular biological methods of five specific recognition elements, including aptamers, genetic engineering antibodies, DNAzymes, genetically engineered enzymes, and whole-cell-based biosensors. In addition, the application of these identification elements combined with biosensor and immunoassay methods in actual detection was also discussed. The purpose of this review was to provide a valuable reference for further development of rapid detection methods for pesticide residues.

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