Development and Immunogenic Evaluation of a Recombinant Vesicular Stomatitis Virus Expressing Nipah Virus F and G Glycoproteins

文献类型： 外文期刊

第一作者： Guo, Huijuan

作者： Guo, Huijuan;Liu, Renqiang;Pan, Dan;Dang, Yijing;Meng, Shuhuai;Shan, Dan;Wang, Xijun;Ge, Jinying;Bu, Zhigao;Wen, Zhiyuan;Bu, Zhigao

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关键词： Nipah virus; recombinant vesicular stomatitis virus; neutralizing antibodies

期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）

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年卷期： 2025 年 17 卷 8 期

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收录情况： SCI

摘要： Nipah virus (NiV) is a highly pathogenic bat-borne zoonotic pathogen that poses a significant threat to human and animal health, with fatality rates exceeding 70% in some outbreaks. Despite its significant public health impact, there are currently no licensed vaccines or specific therapeutics available. Various virological tools-such as reverse genetics systems, replicon particles, VSV-based pseudoviruses, and recombinant Cedar virus chimeras-have been widely used to study the molecular mechanisms of NiV and to support vaccine development. Building upon these platforms, we developed a replication-competent recombinant vesicular stomatitis virus (rVSV Delta G-eGFP-NiVBD F/G) expressing NiV attachment (G) and fusion (F) glycoproteins. This recombinant virus serves as a valuable tool for investigating NiV entry mechanisms, cellular tropism, and immunogenicity. The virus was generated by replacing the VSV G protein with NiV F/G through reverse genetics, and protein incorporation was confirmed via immunofluorescence and electron microscopy. In vitro, the virus exhibited robust replication, characteristic cell tropism, and high viral titers in multiple cell lines. Neutralization assays showed that monoclonal antibodies HENV-26 and HENV-32 effectively neutralized the recombinant virus. Furthermore, immunization of golden hamsters with inactivated rVSV Delta G-eGFP-NiVBD F/G induced potent neutralizing antibody responses, demonstrating its robust immunogenicity. These findings highlight rVSV Delta G-eGFP-NiVBD F/G as an effective platform for NiV research and vaccine development.

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